ID 287
Title Transcription
ガクカセン ニオケル エンショウセイ サイトカイン IL-β ノ プロセッシング
Title Alternative
Processing of IL-1β by Tissue Kallikrein mK13 in the Mouse Submandibular Gland
Author
YAO, Chenjuan Department of Molecular Oral Physiology, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
IL-β
Kallikrein
processing
Content Type
Departmental Bulletin Paper
Description
We have shown earlier that the expression of interleukin-1β (IL-1β) mRNA is increased in the submandibular gland (SMG) after injection of lipopolysaccharide (LPS). In this present study we suggest that tissue kallikrein mK13 is a candidate of the processing enzyme for the precursor of IL-1β (pro-IL-1β) in the SMG. High levels of 17.5- and 20-kDa IL-1β proteins were detected by Western blotting in the SMG, and saliva from LPS-injected mice. Despite this fact, pro-IL-1β with a molecular size of 35-kDa was not detected in this tissue. The protein for IL-1β-converting enzyme (ICE) was expressed only at a low level in the SMG as compared with its level in various epithelial tissues or LPS-stimulated macrophages. We detected the strong expression of members of kallikrein family (mK1, mK9, mK13, and mK22) in the SMG but not other tissues. By incubation with mK13, but not with mK1, mK9, nor mK22, the 35-kDa pro-IL-1β was cleaved to give two major products with molecular masses of 17.5- and 22- kDa, which production was inhibited by PMSF, a serine protease inhibitor, but not by ICE inhibitors. The pro-IL-β peptide segment, which includes the putative processing site, was hydrolyzed at Leu^<113> and Leu^<114> by incubation with mK13. The immunohistochemistry and an autonomic therapy experiment showed that IL-1β and kallikrein mK13 were co-localized in the secretory granules of granular convoluted tubular cells. Our present results thus suggest that kallikrein mK13 is a plausible candidate for the processing enzyme for pro-IL-1β in the SMG of mice.
Journal Title
四国歯学会雑誌
ISSN
09146091
NCID
AN10050046
Volume
19
Issue
2
Start Page
177
End Page
181
Sort Key
177
Published Date
2007-01
Remark
公開日:2010年1月24日で登録したコンテンツは、国立情報学研究所において電子化したものです。
EDB ID
FullText File
language
jpn
departments
Oral Sciences
Report Type
受賞講演