ID 29050
Author
Kuwahara, Tomomi Department of Bacteriology, The University of Tokushima School of Medicine
Nakayama, Haruyuki Department of Bacteriology, The University of Tokushima School of Medicine
Miki, Tsuyosi Department of Bacteriology, The University of Tokushima School of Medicine
Kataoka, Keiko Department of Bacteriology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Arimochi, Hideki Department of Bacteriology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Ohnishi, Yoshinari Department of Bacteriology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory
Keywords
Bacteroides fragilis
neuraminidase
oligonucleotide probe
septicemia
PCR
Content Type
Journal Article
Description
Bacteroides fragilis is a Gram-negative obligate anaerobe frequently isolated from clinical specimens and sometimes causes severe septicemia in compromised hosts. Increasing interest has been shown in the enterotoxigenicity and drug resistance of B. fragilis in the field of medical microbiology. We previously reported rapid detection of this anaerobe by nested PCR targeting a neuraminidase-encoding gene nanH. In the present study, we synthesized a digoxigenin-labeled oligonucleotide probe, NH1,which is specific for nanH of B. fragilis, and we combined the hybridization assay using NH1with the nanH-PCR to detect this anaerobe in a bacteremia model mice. In the specificity test, the oligonucleotide probe, NH1, hybridized only to amplification products from B. fragilis. PCR-dot blot hybridization based on nanH enabled detection of cells of B. fragilis in blood samples even when the number was as low as 2x103colony-forming units/ml. These findings suggest that PCR-dot blot hybridization targeting nanH is a useful procedure for diagnosis of septicemia caused by B. fragilis when viable cells in blood cannot be detected by the traditional culture techniques.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
48
Issue
1-2
Start Page
60
End Page
65
Sort Key
60
Published Date
2001
Remark
EDB ID
FullText File
language
eng
departments
Medical Sciences