ID 29051
Author
Chiampanichayakul, Sawitree Department of Bacteriology, The University of Tokushima School of Medicine|Department of Biochemistry, Faculty of Medicine, Chiang Mai University
Kataoka, Keiko Department of Bacteriology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Arimochi, Hideki Department of Bacteriology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Thumvijit, Somsakul Department of Bacteriology, The University of Tokushima School of Medicine|Department of Biochemistry, Faculty of Medicine, Chiang Mai University
Kuwahara, Tomomi Department of Bacteriology, The University of Tokushima School of Medicine
Nakayama, Haruyuki Department of Bacteriology, The University of Tokushima School of Medicine
Vinitketkumnuen, Usanee Department of Biochemistry, Faculty of Medicine, Chiang Mai University
Ohnishi, Yoshinari Department of Bacteriology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory
Keywords
bitter melon
Momordica charantia Linn
antimutagenicity
azoxymethane-induced aberrant crypt foci
O6-methylguanine
Content Type
Journal Article
Description
Antimutagenicity and chemopreventive activity of an 80%-ethanol extract of bitter melon (Momordica charantia Linn.) against the formation of azoxymethane (AOM)-induced aberrant crypt foci (ACF) was investigated. The bitter melon extract was nonmutagenic and inhibited the mutagenicity of heterocyclic amines 2-amino-3,4-dimethylimidazo[4,5-f]quinoline and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine, and aflatoxin B1 in the Salmonella mutation assay. To examine the inhibitory effect of bitter melon on AOM-induced ACF formation, male F344 rats were fed various concentrations of the extract (0.1, 0.5, and 1.0 g/kg body weight) for five weeks during the initiation stage. One week after the administration of the plant extract, rats were subcutaneously given AOM at 15 mg/kg body weight once a week for two weeks. Three rats in each group were sacrificed 12 hr after the second AOM injection to analyze DNA adducts, O6-methylguanine (O6-meG) and N7-methylguanine in the liver and colon. The remaining rats were sacrificed 3 weeks after the second AOM injection to observe ACF. To examine the inhibitory effect of the extract on ACF formation in the postinitiation stage, rats were fed the extract at 0.1 and 1.0 g/kg body weight for 12 weeks starting two weeks after the second AOM injection. Treatment with bitter melon extract significantly inhibited ACF formation in the colon during the initiation stage and dose-dependently decreased the average of O6-meG DNA adduct in the colonic mucosa. During the postinitiation stage, bitter melon extract, at 1.0 g/kg body weight, significantly inhibited ACF formation in the colon, especially the formation of ACF with four or more crypts per focus. These findings suggest that bitter melon is a possible chemopreventive agent against colon carcinogenesis.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
48
Issue
1-2
Start Page
88
End Page
96
Sort Key
88
Published Date
2001
Remark
EDB ID
FullText File
language
eng
departments
Medical Sciences