Sawada, Kazuhiko Department of Anatomy, The University of Tokushima School of Medicine
Fukui, Yoshihiro Department of Anatomy, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
rolling mouse Nagoya
This report describes recent studies on tyrosine hydroxylase (TH) expression in Purkinje cells of the cerebellum of ataxic mutant mice. An increased expression of TH in some Purkinje cells has been observed in two allelic groups of mutant mice, tottering and dilute. TH-positive Purkinje cells appeared preceding the onset of ataxia. Northern blot analysis revealed2.1kb of TH mRNA in the mutant cerebella, and the size was identical to that of TH transcripts in other brain regions. However, TH in Purkinje cells did not seem to participate in catecholamine biosynthesis. In vitro studies showed that cultured non-catecholaminergic neurons expressed the TH transcripts following Ca2+ influx. Therefore, abnormal TH expression in the mutant Purkinje cells may indicate neuronal dysfunction caused by misregulation of intracellular Ca2+concentrations.
The journal of medical investigation : JMI
LID201112012001.pdf 210 KB