ID 29078
Author
Dainichi, Teruki Department of Parasitology and Immunology, The University of Tokushima School of Medicine
Maekawa, Yoichi Department of Parasitology and Immunology, The University of Tokushima School of Medicine
Ishii, Kazunari Department of Parasitology and Immunology, The University of Tokushima School of Medicine
Himeno, Kunisuke Department of Parasitology and Immunology, The University of Tokushima School of Medicine
Keywords
Cystatin
Nippostrongylus brasiliensis
cloning
Content Type
Journal Article
Description
A novel member of the cystatin family, nippocystatin (NbCys), was identified from excretory-secretory (ES)-products of a nematode Nippostrongylus brasiliensis, and the cDNA was cloned and sequenced. The mRNA of NbCys was confirmed to be expressed in both larvae and adults of the parasite. NbCys was translated as a proform with a single domain for secretion and was detected as a 14-kDa mature form in ES-products of the adult worm. Recombinant protein of NbCys profoundly inhibited the activity of cysteine proteases such as cathepsin L and B, but not that of cathepsin D, an aspartic protease. Furthermore, the ES-products had also been confirmed to inhibit cysteine proteases. Taken together, NbCys may play a role in evasion of N. brasiliensis from host defense systems, since cysteine proteases are known to participate in immune systems of infected hosts.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
48
Issue
1-2
Start Page
81
End Page
87
Sort Key
81
Published Date
2001
Remark
EDB ID
FullText File
language
eng