ID 30592
Author
Kawahara, Tsukasa Department of Nutritional Physiology, The University of Tokushima School of Medicine
Kuwano, Yuki Department of Nutritional Physiology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kondo, Shigetada Department of Nutritional Physiology, The University of Tokushima School of Medicine KAKEN Search Researchers
Kawai, Tomoko Department of Nutritional Physiology, The University of Tokushima School of Medicine
Nikawa, Takeshi Department of Nutritional Physiology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kishi, Kyoichi Department of Nutritional Physiology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Rokutan, Kazuhito Department of Nutritional Physiology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
toll-like receptor
gastric pit cell
Helicobacter pylori
mitogen oxidase1
Content Type
Journal Article
Description
Gastric pit cells express mitogen oxidase1 (Mox1) and essential components for the phogocyte NADPH oxidase (p67-, p47-, p40-, and p22-phoxes). Helicobacter pylori (Hp) lipopolysaccharide (LPS) is a potent up-regulator of the Mox1 oxidase. In this study, we examined the expression levels of several key members of the Toll-like receptor (TLR) family in primary cultures of guinea pig gastric pit cells. These cells expressed the TLR4 mRNA. Immunoblot analysis and immunofluorescence histochemistry with an anti-TLR4 antibody showed that gastric pit cells possessed significant amounts of TLR4 protein preferentially on the plasma membrane. In contrast, the cells did not express the TLR2 and TLR9 transcripts and did not contain detectable amounts of TLR2 protein. Neither peptidoglycan from Staphylococcus aureus nor Hp DNA with the CpG motif up-regulated Mox1 oxidase activity. Hp LPS activated nuclear factor-κB in association with the expression of cyclooxygenase II and tumor necrosis factor α transcripts. These findings suggest that TLR4 may play a crucial role in the initiation of inflammatory responses of gastric pit cells against Hp infection.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
48
Issue
3-4
Start Page
190
End Page
197
Sort Key
190
Published Date
2001
Remark
EDB ID
FullText File
language
eng
departments
Medical Sciences