ID 312
Title Transcription
シシュウ ビョウヘン キョクショ ニオケル ジョウヒ サイボウカン セッチャク キコウ ニ カンヨ スル ブンシグン ノ ハツゲン カイセキ
Title Alternative
The Analysis of Expression Pattern of Claudins Family, E-cadherin, JAM-A and Occludin in Periodontal Diseases
Author
Yoneda, Miho Department of Conservative Dentistry, Graduate School of Dentistry, The University of Tokushima
Keywords
歯周病
密着結合
クローディン
上皮細胞
Content Type
Departmental Bulletin Paper
Description
Tight junctions form the apical junctional complex in the epithelium and are essential for the tight sealing of cellular sheets. They are composed of three major integral membrane proteins, i.e., occludin, claudins, and junctional adhesion molecules. Expression patterns of claudins are tissue specific and most tissues express multiple claudins. The combination of claudin proteins is thought to determine the strength of the tight junctions of the tissue ; however, the expression pattern of the claudin family in human gingival epithelium remains unclear. The aim of this study was to examine the expression pattern of the claudin family, E-cadherin, JAM-A and occludin, in chhronic inflammatory lesions of gingival epithelium. Expression levels of mRNA in human gingival tissue from healthy gingivae and inflammation gingivae (probing depth>6mm, sulcular bleeding on probing) were analyzed by RT-PCR. To determine the expression patterns of tight junction molecules in situ, claudin-10, E-cadherin and JAM-A was detected by immunohistochemistry and in situ hybridization. Sa3 (squamous carcinoma cell derived from human oral cancer) was cultured in the monolayer in the presence or absence of TNF-α and IL-13, and the expression of the claudin family and other TJ-related protein were detected by RT-PCR and flow cytometry. To confirm whether inflammatory cytokines, TNF-α and IL-13, affect the functional integrity of Sa3 cell layers, transepithelial electrical resistance (TER) and transepithelial cell permeability were measured. RT-PCR analysis showed that claudin-1, -4, -7 and-12 were expressed but claudin-2, -6, -9 and -11 were not expressed in human normal gingival tissues. Of particular significance, the expression level of claudin-10 was increased in inflamed gingival tissues compared with normal gingival tissues. In situ hybridization and immunohistochemical analysis showed that claudin-10 expression was strongly detected in the basal layer of the epithelium in inflamed gingival tissues. In a monolayer culture of Sa3, TNF-α and IL-13 decreased the mRNA expression levels of E-cadherin, occludin and JAM-A in a dose-dependent manner, and increased those of claudin-2, 3, 4 and 8. In addition, TNF-α and IL-13 decreased TER and increased the transepithelial cell permeability of monolayer Sa3 cells. These data suggest that periodontal inflammatory response induces claudin-10 expression and leads to modification of the function of gingival epithelium. This alteration in inflamed gingival tissues may contribute to the pathogenesis of periodontitis.
Journal Title
四国歯学会雑誌
ISSN
09146091
NCID
AN10050046
Volume
20
Issue
1
Start Page
89
End Page
103
Sort Key
89
Published Date
2007-06
Remark
公開日:2010年1月24日で登録したコンテンツは、国立情報学研究所において電子化したものです。
FullText File
language
jpn
Report Type
学位論文