ID 61874
Title Alternative
プラシノ藻類Tetraselmis tetratheleにおける水銀処理による酸化ストレスについて
Author
Matsumoto, Yoshiyuki University of Tokushima
Keywords
cytotoxicity
H2O2
mercury
oxidative stress
Prasinophyceae
Tetraselmis tetrathele
Content Type
Departmental Bulletin Paper
Description
Mercury-induced oxidative stress was observed in the cells of marine phytoplankton Tetraselmis tetrathele
(Prasinophyceae) using the fluorescent probes 2' 7-dichlorofluorescindiacetate (DCFH-DA) and fluorescein
diacetate (FDA). When the cells were exposed to 1μM of HgCl2, the fluorescence intensity of 2'
7' -dichlorofluorescin (DCF) was three times higher than that of the control level 60 min after the treatment,
indicating H202 accumulation,and it gradually decreased. Moreover,exposure to 0.3μM of HgCl2 caused an
increase in fluorescence 6h after the treatment. However,exposure to HgCl2 at concentrations of 3.0μM or
greater did not cause an increase in fluorescence. It is thought that H202 was also generated at these
concentrations,but could not be detected due to inactivation of the cellular esterase necessary to convert
DCFH-DA to DCFH,and/or leakage from the cells due to membrane damage caused by HgCl2. The maximum
level of fluorescence was approximately two times higher under light conditions than under dark conditions,
suggesting that H202 was derived partly from photosynthesis. These results indicate that accumulation of H202 is
involved inconsequence of the toxicity of mercury.
Journal Title
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
ISSN
09146385
NCID
AN10065859
Publisher
徳島大学総合科学部
Volume
22
Start Page
57
End Page
63
Sort Key
57
Published Date
2008-12
EDB ID
FullText File
language
eng
departments
Bioscience and Bioindustry