ID 63019
Author
Akiyama, Jun University of Tokushima
Ogasa, Hiroshi University of Tokushima
Hirata, Yoshiko University of Tokushima
Yamashita, Kohei University of Tokushima
Shibagaki, Haruka University of Tokushima
Tanaka, Fumika University of Tokushima
Sakanashi, Yoko University of Tokushima
Keywords
econazole
calcium
cytotoxicity
lymphocyte
Content Type
Departmental Bulletin Paper
Description
Econazole, one of azole antifungals, is proven to exhibit an inhibitory action on Mycobaterium
tuberculosis and its multidrug-resistant strains under in vitro and ex vivo conditions. However, econazole has been used as a pharmacological tool for inhibiting capacitative Ca2+ influx and exerts multiple effects on cellular Ca2+ circumstance. Therefore, to suggest the toxic effect of econazole at therapeutic concentrations, we have tested on the effect on membrane Ca2+ transport in rat thymocytes by using a flow cytometer with Fluo-3, an indicator of intracellular Ca2+. Econazole at concentrations of 1-3 μM increased membrane Ca2+ permeability and inhibited capacitative Ca2+ influx without affecting passive Ca2+ influx, Ca2+ release from intracellular Ca2+
store sites, and membrane Ca2+ pump. Econazole at 0.3 μM, a therapeutic concentration against tuberculosis caused by multidrug-resistant and latent M. tuberculosis, did not affect membrane Ca2+ transport. It may be suggested that econazole at therapeutic concentrations exerts no side effect related to Ca2+.
Journal Title
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
ISSN
09146385
NCID
AN10065859
Publisher
徳島大学総合科学部
Volume
23
Issue
1
Start Page
1
End Page
6
Sort Key
1
Published Date
2009-03-25
EDB ID
FullText File
language
eng
departments
Bioscience and Bioindustry