ID 63727
Author
Matsui, Hiroko Kyushu University
Sakanashi, Yoko Kyushu University
Nishimura, Yumiko Okayama University
Kawanai, Takuya University of Tokushima
Ishida, Shiro Tokushima Bunri University
Okano, Yoshiro Tokushima Bunri University
Keywords
TPEN
DTPA
zinc
hydrogen peroxide
cytotoxicity
Content Type
Departmental Bulletin Paper
Description
Recently, we have revealed that trace Zn2+ partly attenuates Ca2+-dependent cell death induced by A23187, a calcium ionophore, in rat thymocytes. In this study, to see if Zn2+ attenuates the H2O2-induced cell death that is also Ca2+-dependent, the effects of ZnCl2 and chelators for Zn2+ have been examined by using a flowcytometer with propidium iodide. The incubation with H2O2 increased the cell lethality. Simultaneous
application of ZnCl2 greatly augmented the H2O2-induced increase in lethality. DTPA, a chelator for extracellular Zn2+, did not affect the increase in cell lethality by H2O2. However, the H2O2-induced increase in cell lethality was greatly attenuated by TPEN, a chelator for extracellular and intracellular Zn2+. Taken together, it may be likely that intracellular Zn2+ modifies the H2O2-induced cytotoxicity. However, it cannot be ruled out the possibility that TPEN chelates intracellular Fe2+, resulting in inhibiting the formation of hydroxyl radical
from H2O2 that leads to an attenuation of H2O2 cytotoxicity.
Journal Title
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
ISSN
09146385
NCID
AN10065859
Publisher
徳島大学総合科学部
Volume
23
Issue
2
Start Page
12
End Page
18
Sort Key
12
Published Date
2009-07-01
EDB ID
FullText File
language
eng
departments
Bioscience and Bioindustry