ID 69789
Author
Kawanai, Takuya Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushima
Harada, Soya Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushima
Kubota, Chiharu Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushima
Hashimoto, Erika Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushima
Oyama, Yasuo Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
Zinc
Vitamin C
Hydrogen peroxide
Cytotoxicity
Content Type
Departmental Bulletin Paper
Description
The ‘antioxidant hypothesis’ proposes that antioxidant nutrients afford protection against chronic diseases by
decreasing oxidative damages. The ability of zinc to retard oxidative processes has been recognized for many
years. However, the application of ZnCl2 potentiates the cytotoxicity of H2O2. Thus, some antioxidants may
be cytotoxic under certain oxidative conditions. Therefore, in this study, the effect of vitamin C, one of
antioxidant nutrients, on the cells treated with H2O2 has been examined to see if vitamin C potentiates the
cytotoxicity of H2O2. Experiments were carried out with flow cytometer and rat thymocytes. Vitamin C also
potentiated the cytotoxicity of H2O2. The increase in cell lethality induced by the combination of H2O2 and
ZnCl2 was associated with the increase in population of shrunken cells with increased intensity of side scatter.
However, it was not the case for the combination of H2O2 and vitamin C. The profile of cytotoxicity induced
by H2O2 and vitamin C was different from that by H2O2 and ZnCl2. It may be suggested that the effects of zinc
and vitamin C varies from cytoprotective to cytotoxic, being dependent on the type of oxidative stress.
Journal Title
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
ISSN
09146385
NCID
AN10065859
Volume
24
Issue
3
Start Page
12
End Page
19
Sort Key
12
Published Date
2010-07-20
EDB ID
FullText File
language
eng
departments
Bioscience and Bioindustry