ID 74430
Title Transcription
ビセイブツ オ ユライ ト スル キノウセイ タンパクシツ ノ イヨウ コウガクテキ オウヨウ ニ カンスル ケンキュウ
Title Alternative
A Study on Functional Protein Tool Derived from Bacterial Products for Clinical Engineering Application
Author
Tabata, Atsushi Department of Biological Science and Technology,Life System, Institute of Technology and Science, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Sakakura, Eriko Graduate School of Advanced Technology and Science, The University of Tokushima
Tomoyasu, Toshifumi Department of Biological Science and Technology,Life System, Institute of Technology and Science, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Nagamune, Hideaki Department of Biological Science and Technology,Life System, Institute of Technology and Science, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
Exotoxin A
Functional Protein Tool
Clinical Engineering Application
ScFv
Bacterial Protein Toxin
Content Type
Departmental Bulletin Paper
Description
A study on novel functional protein tool derived from bacterial products was carried out. This
tool is composed of two parts, a functional domain to exhibit regulated cytotoxicy and a targeting
domain to recognize the target cell, and these domains are connected with a chemical linker. A
Pseudomonas aeruginosa exotoxin A (ETA) deficient in its receptor-binding domain, named
ΔBD-ETA, was adopted as the functional domain. ΔBD-ETA was expressed in Escherichia coli
expression system and purified by Ni-NTA affinity chromatography. Purified ΔBD-ETA indicates no
cytotoxity to human lung carcinoma A549 at the concentrations showing severe cytotoxity of
wild-type ETA. A recombinant single chain Fv (ScFv) derived from anti-carcinoembryonic
antigen (CEA) antibody, named anti-CEA-ScFv, was selected as the targeting domain. In contrast
to ΔBD-ETA, anti-CEA-ScFv was difficult to express stably as functional targeting domain against
CEA. Thus, the construction of functional protein tool has not been completed at the moment.
However, further attempts to overcome the problem including the preparation of
alternative/improved functional protein tool are in progress.
Journal Title
徳島大学大学院ソシオテクノサイエンス研究部研究報告
ISSN
21859094
NCID
AA12214889
Volume
55
Start Page
51
End Page
58
Sort Key
51
Published Date
2010
FullText File
language
jpn
departments
Bioscience and Bioindustry