ID 74520
Title Transcription
イガクテキ オウヨウ ニ ムケタ サイキン ドクソ ニ ユライ スル キノウセイ ナノ バイオ ツール ノ サクセイ
Title Alternative
Development of Functional Nano-Bio Tool Derived from Bacterial Protein Toxin for Clinical Application
Author
Tabata, Atsushi Department of Biological Science and Technology,Life System, Institute of Technology and Science, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Sakakura, Eriko Graduate School of Advanced Technology and Science, The University of Tokushima
Tomoyasu, Toshifumi Department of Biological Science and Technology,Life System, Institute of Technology and Science, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Nagamune, Hideaki Department of Biological Science and Technology,Life System, Institute of Technology and Science, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
Cholesterol-Dependent Cytolysin
Functional Protein Tool
Drug Delivery System
Clinical Application
Bacterial Protein Toxin
Content Type
Departmental Bulletin Paper
Description
A novel functional protein tool derived from cholesterol-dependent cytolysin was developed and
its application for clinical field such as a drug delivery system was investigated. This tool is
composed of both of a functional-domain to connect the drug carrier and a targeting-domain to
recognize the target cell. The recombinant protein consisted of a lung tumor specific peptide,
domain 1-3 of intermedilysin with an introduced disulphide-bond to block the conformational
change and pore-formation, and domain 4 of Streptococcus mitis-derived human platelet
aggregation factor, was prepared using bacterial-expression system then investigated its function. It
was shown that the prepared protein tool could specifically deliver a model of drug-carrier vesicles
to human lung tumor cells in vitro. This result suggests that the novel functional protein tool
constructed in this study has the superior properties for clinical application.
Journal Title
徳島大学大学院ソシオテクノサイエンス研究部研究報告
ISSN
21859094
NCID
AA12214889
Volume
54
Start Page
51
End Page
56
Sort Key
51
Published Date
2009
FullText File
language
jpn
departments
Bioscience and Bioindustry