ID 83260
Author
Tsuji, Masahito Department of Urology, The University of Tokushima School of Medicine
Kanda, Kazuya Department of Urology, The University of Tokushima School of Medicine
Murakami, Yoshihide Department of Urology, The University of Tokushima School of Medicine
Kurokawa, Yasushi Department of Urology, The University of Tokushima School of Medicine
Kanayama, Hiro-omi Department of Urology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Sano, Toshiaki First Department of Pathology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kagawa, Susumu Department of Urology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
prostatic intraepithelial neoplasia
mmunohistochemistry
fluorescence in situ hybridization
Content Type
Journal Article
Description
Objective:We evaluated the biological properties of High-grade prostatic intraepithelial neoplasia (PIN) by immunohistochemistry and fluorescence in situ hybridization (FISH) analysis in relation to normal tissue and carcinoma lesions.
Materials and Methods:Immunohistochemical staining and FISH were performed on23formalin-fixed radical prostatectomy specimens taken from patients with PIN. Assays were performed using MIB-1, chromogranin A (CGA) and an anti-androgen receptor antibody (AR). A centromere probe for chromosome8was used to test for aneuploidy.
Results:The MIB-1index of cancerous specimens (16.2±10.5%) was significantly higher than that of benign (1.9±1.6%, p<0.0001) or PIN (4.0±4.5%, p<0.0001) specimens. The percentage of CGA positive cells was significantly lower in normal tissue (1.2±1.8%) than in PIN (3.5±2.9%, p=0.012) or carcinoma (5.4±4.9%, p=0.005) lesions. Positive staining for AR was consistently observed in the nuclei of both benign and malignant epithelial cells, but positive cytoplasmic staining was also seen in PIN epithelial cells. No significant difference in FISH detected anomalies were found between PIN and carcinoma specimens.
Conclusions:Our studies concerning proliferative activity, NE differentiation and chromosomal anomalies of prostatic specimens support the hypothesis that PIN is a biologically intermediate stage in the pathogenesis of prostatic carcinoma. The cellular distribution of AR was altered in PIN cells, but the role of AR in PIN is not yet clear.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
46
Issue
1-2
Start Page
35
End Page
41
Sort Key
35
Published Date
1999
Remark
FullText File
language
eng
departments
Medical Sciences