ID 83488
Author
Hayashi, Shigehito Second Department of Internal Medicine, The University of Tokushima School of Medicine
Muguruma, Naoki Second Department of Internal Medicine, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Bando, Terumi Second Department of Internal Medicine, The University of Tokushima School of Medicine
Taoka, Satoko Second Department of Internal Medicine, The University of Tokushima School of Medicine
Ito, Susumu Second Department of Internal Medicine, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory
Ii, Kunio First Department of Pathology, The University of Tokushima School of Medicine
Keywords
micro-cancer
diagnostic method
indocyanine green (ICG) derivatives
vital immunohistochemical staining
Content Type
Journal Article
Description
It becomes possible to establish a novel diagnostic method for micro-cancer by modulating the signals from the lesion, if lesions can be labeled with substances which can be detected by video endoscopy. The authors have already succeeded in synthesizing indocyanine green (ICG) derivatives for a fluorescent labeling substance which emits near-infrared rays. Before the antibodies labeled by these substances can be used, it is necessary to establish a method of vital immunohistochemical staining. So, we inves-tigated the responses of antibodies exposed to non-fixed fresh tissue specimens as a basic study on vital immunohistochemical staining. The responses of fresh esophageal and gastric specimens (biopsied or surgically resected) to immunohistochemical staining with anti-epithelial membrane antigen (EMA) antibodies under various conditions using the ABC method were examined. These tissue specimens were stained immunohistochemically, and incubated with anti-EMA antibodies for 10 and 30 minutes (esophagus), and for 60 and 120 minutes (stomach) at 37°C. These results suggest that vital immunohistochemical staining is possible under optimum conditions. If an infrared fluorescent endoscopy catching this excited fluorescence can be developed, it will be possible to establish a new endoscopic diagnostic method on the basis of vital immunohistochemical staining.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
46
Issue
3-4
Start Page
178
End Page
185
Sort Key
178
Published Date
1999
Remark
EDB ID
FullText File
language
eng
departments
Medical Sciences