ID 83933
Author
Iwahashi, Shuichi Department of Digestive Surgery and Transplantation, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Ishibashi, Hiroki Department of Digestive Surgery and Transplantation, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Utsunomiya, Tohru Department of Digestive Surgery and Transplantation, Institute of Health Biosciences, the University of Tokushima Graduate School
Morine, Yuji Department of Digestive Surgery and Transplantation, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Ochir, Lkhaguva Tovuu Department of Digestive Surgery and Transplantation, Institute of Health Biosciences, the University of Tokushima Graduate School
Hanaoka, Jun Department of Digestive Surgery and Transplantation, Institute of Health Biosciences, the University of Tokushima Graduate School
Mori, Hiroki Department of Digestive Surgery and Transplantation, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Ikemoto, Tetsuya Department of Digestive Surgery and Transplantation, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Imura, Satoru Department of Digestive Surgery and Transplantation, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Shimada, Mitsuo Department of Digestive Surgery and Transplantation, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
pancreas cancer
cholangiocarcinoma
HDAC inhibitor
valproic acid
epigenetic regulation
Content Type
Journal Article
Description
Background : Histone deacetylase (HDAC) is well known to be associated with
tumorigenesis through epigenetic regulation, and its inhibitors (HDACIs) induce differentiation
and apoptosis of tumor cells. We examined the therapeutic effects of valproic
acid (VPA, a HDACI) with a combination of 5-fluorouracil (5-FU) in vitro. Methods : A human
pancreas cancer cell line (SUIT-2) and a cholangiocarcinoma cell line (HuCCT1) were
used. Cell viabilities were evaluated by a cell proliferation assay. We determined the anticancer
effects of VPA combined with 5-FU in these cell lines. Results : Pancreas cancer
(SUIT-2) : No effect of 5-FU (1.0 μM) was observed, but 17% and 30% of proliferationinhibitory
effects were recognized in a dose of 2.5 or 5.0 μM, respectively. Cell viability
was only weakly reduced by VPA (0.5 mM). However, in combination of 5-FU (1.0 μM)
with VPA (0.5 mM), 19% of inhibitory effect was observed. Cholangiocarcinoma (HuCCT1) :
5-FU (1.0 μM) did not suppress the cell viability, but 5-FU (2.5 μM) suppressed by 23%.
VPA (0.5 mM) did not suppress the cell viability, while VPA (1.0 mM) weakly decreased it
by 11%. Combination of 5-FU (1.0 μM) and VPA (0.5 mM) markedly reduced the cell viability
by 30%. Conclusion : VPA augmented the anti-tumor effects of 5-FU in cancer cell
lines. Therefore, a combination therapy of 5-FU plus VPA may be a promising therapeutic
option for patients with pancreas cancer and cholangiocarcinoma.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
58
Issue
1-2
Start Page
106
End Page
109
Sort Key
106
Published Date
2011-02
Remark
The journal of medical investigation : http://medical.med.tokushima-u.ac.jp/jmi/index.html
EDB ID
FullText File
language
eng
departments
University Hospital
Medical Sciences