ゴーヤ シュシ チュウシュツ コウ H レクチン ノ リンショウ ケンサ エノ オウヨウ
Preparation method for a specific anti-H lectin isolated from goya seeds and its application in clinical laboratories
Aki, Kensaku Subdivision of Biomedical Laboratory Sciences, Graduate School of Health Sciences, the University of Tokushima|Department of Cells and Immunity Analytics, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Aihara, Minako National Hospital Organization Kyoto Medical Center
Moriuchi, Takako National Hospital Organization Kyoto Medical Center
Mori, Miwa Department of Biochemistry, National Cerebral and Cardiovascular Center Research Institute
Hosoi, Eiji Department of Cells and Immunity Analytics, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
ABO blood typing
After the discovery of the phenomenon of aggregation of red blood cells with castor bean extract by Peter Hermann Stillmark in１８８８, various plant hemagglutinins were discovered from leguminous plant seed extracts. These plant hemagglutinins were called“lectins”by William C. Boyd et al . Lectins are sugar-binding proteins that are highly specific for their sugar moieties, physiological activity, and hemagglutinating activity. In particular, lectins displaying blood-group specificity are important for blood-group typing and antigen recognition. In recent years, Ulex lectin（anti-H lectin）extracted from the Ulex europaeus seed was used to subdivide blood of type O, and the subgroup of ABO, but Ulex europaeus is now difficult to obtain, so it is necessary to buy expensive reagents.
On the other hand, Momordica charantias （goya）seed extract（goya lectin）was recently revealed to show higher anti-H activity than Ulex lectin. Goya is readily available and inexpensive to obtain. Therefore, we examined a simple method for the preparation of goya lectin.
As a result, we were able to establish a simple method for preparing goya lectin isolated from goya seeds, and confirm its stability against long-term storage and temperature and usefulness as an anti-H lectin. We expect this method to be used in clinical practice in the future.
LID201207092010.pdf 1.41 MB