ID 96890
Author
Kanbara, Yasuhiro Faculty of Integrated Arts and Sciences, The University of Tokushima
Seo, Hakaru Faculty of Pharmaceutical Sciences, Sojo University
Nishimura, Yumiko Dental Department, Hiroshima University Hospital
Oyama, Yasuo Faculty of Integrated Arts and Sciences, The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
Propranolol
Intracellular Zn²⁺level
FluoZin-3
Content Type
Departmental Bulletin Paper
Description
Propranolol is a representative β-blocker used for the treatment of cardiovascular diseases. Because the use
of propranolol expands for some clinical purposes that are not related to its β-blocking action, it is necessary to
further examine cellular actions of propranolol. We revealed that propranolol decreased the intracellular Zn2+
level in rat thymocytes by the use of cytometric technique with FluoZin-3, a fluorescent indicator of intracellular
Zn2+. Propranolol decreased the influx of extracellular Zn2+. However, the agent decreased the intracellular
Zn2+ level even in the presence of DTPA, a chelator of extracellular Zn2+. Thus, the decrease in intracellular
Zn2+ level by propranolol was not due to the decrease in Zn2+ influx by propranolol. Propranolol increased the
cellular content of nonprotein thiol that was estimated by the use of 5-chloromethylfluorescein fluorescence, an
indicator for non-proteinous thiol. Since non-proteinous thiols can make a complex with Zn2+, propranolol may
increase the cellular content of nonprotein thiol, resulting in the decrease in intracellular Zn2+ level. Since the
concentrations of propranolol to affect both intracellular Zn2+ level and cellular content of nonprotein thiol are
higher than those reported under clinical conditions, it is difficult to emphasize clinical implications from present
results.
Journal Title
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
ISSN
09146385
NCID
AN10065859
Volume
26
Issue
3
Start Page
11
End Page
16
Sort Key
11
Published Date
2012-07
EDB ID
FullText File
language
eng
departments
Bioscience and Bioindustry