ID | 74502 |
Title Alternative | 低酸素細胞特異的な蛍光イメージング剤の開発
|
Author |
Nakata, Eiji
Division of Bioinformatics Engineering, Department of Life System, Institute of Technology and Science, Graduate School of the University of Tokushima
Yukimachi, Yoshihiro
Graduate School of Advanced Technology and Science, The University of Tokushima
Abe, Chiaki
Graduate School of Advanced Technology and Science, The University of Tokushima
Uto, Yoshihiro
Division of Bioinformatics Engineering, Department of Life System, Institute of Technology and Science, Graduate School of the University of Tokushima
Tokushima University Educator and Researcher Directory
KAKEN Search Researchers
Hori, Hitoshi
Division of Bioinformatics Engineering, Department of Life System, Institute of Technology and Science, Graduate School of the University of Tokushima
Tokushima University Educator and Researcher Directory
KAKEN Search Researchers
|
Keywords | Fluorescent pH probe
Tumor hypoxia
Fluorescent imaging
Bioreductive activation
Nitro reduction
|
Content Type |
Departmental Bulletin Paper
|
Description | We have designed and evaluated UTX-12 as a novel fluorescent pH probe for tumor hypoxia
imaging. UTX-12 consists of a p-nitro benzyl moiety, which is a latent hypoxia-selective leaving group activated by nitro reduction, directly linked to SNARF. Although UTX-12 itself is colorless and non-fluorescent in aqueous solution, nitro reduction triggers the release of SNARF which has well-characterized long wavelength absorption and fluorescence that is sensitive to pH. The resultant SNARF, released intracellularly by enzymatic reduction of UTX-12, allows measurement of pH by pH-dependent dual emission shifts. UTX-12 showed clear differences in fluorescence behavior between hypoxic and aerobic conditions in liver microsomes and inside V79 cells. These data are confirmation that UTX-12 is biologically reduced inside tumor cells and the released SNARF should monitor intracellular pH of tumor cells selectively with reduced background signal. |
Journal Title |
徳島大学大学院ソシオテクノサイエンス研究部研究報告
|
ISSN | 21859094
|
NCID | AA12214889
|
Volume | 55
|
Published Date | 2010
|
FullText File | |
language |
eng
|
departments |
Bioscience and Bioindustry
|