ID 113344
Author
Kikuchi, Yusaku The University of Tokushima
Furukawa, Kazuhiro The University of Tokushima KAKEN Search Researchers
Keywords
Oligonucleotide
2'-Modified-4'-thioRNA
Gene suppression
U1 snRNA interference
Content Type
Journal Article
Description
Gene suppression via U1 small nuclear RNA interference (U1i) is considered to be one of the most attractive approaches, and takes the place of general antisense, RNA interference (RNAi), and anti-micro RNA machineries. Since the U1i can be induced by short oligonucleotides (ONs), namely U1 adaptors consisting of a ‘target domain’ and a ‘U1 domain’, we prepared adaptor ONs using 2'-modified-4'-thionucleosides developed by our group, and evaluated their U1i activity. As a result, the desired gene suppression via U1i was observed in ONs prepared as a combination of 2'-fluoro-4'-thionucleoside and 2'-fluoronucleoside units as well as only 2'-fluoronucleoside units, while those prepared as combination of 2'-OMe nucleoside/2'-OMe-4'-thionucleoside and 2'-fluoronucleoside units did not show significant activity. Measurement of Tm values indicated that a higher hybridization ability of adaptor ONs with complementary RNA is one of the important factors to show potent U1i activity.
Journal Title
Bioorganic & Medicinal Chemistry
ISSN
09680896
NCID
AA10938083
AA11522681
Publisher
Elsevier
Volume
21
Issue
17
Start Page
5292
End Page
5296
Published Date
2013-06-18
Rights
©2013 The Authors. Published by Elsevier Ltd.Open access under CC BY-NC-ND license.(https://creativecommons.org/licenses/by-nc-nd/3.0/)
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Pharmaceutical Sciences