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ID 114747
Author
Nagano, Hikaru Tokushima University|Sagami Women’s University
Yamagishi, Naoko Tokushima University
Tomida, Chisato Tokushima University
Yano, Chiaki Tokushima University
Aibara, Kana Tokushima University
Kohno, Shohei Tokushima University
Okumura, Yuushi Sagami Women’s University KAKEN Search Researchers
Mills, Edward M University of Texas at Austin
Keywords
Bifunctional RNA
Insulin receptor substrate-1
Myogenic differentiation
Rb
Content Type
Journal Article
Description
Background: There is evidence that several messenger RNAs (mRNAs) are bifunctional RNAs, i.e. RNA transcript carrying both protein-coding capacity and activity as functional non-coding RNA via 5′ and 3′ untranslated regions (UTRs).
Results: In this study, we identified a novel bifunctional RNA that is transcribed from insulin receptor substrate-1 (Irs-1) gene with full-length 5′UTR sequence (FL-Irs-1 mRNA). FL-Irs-1 mRNA was highly expressed only in skeletal muscle tissue. In cultured skeletal muscle C2C12 cells, the FL-Irs-1 transcript functioned as a bifunctional mRNA. The FL-Irs-1 transcript produced IRS-1 protein during differentiation of myoblasts into myotubes; however, this transcript functioned as a regulatory RNA in proliferating myoblasts. The FL-Irs-1 5′UTR contains a partial complementary sequence to Rb mRNA, which is a critical factor for myogenic differentiation. The overexpression of the 5′UTR markedly reduced Rb mRNA expression, and this reduction was fully dependent on the complementary element and was not compensated by IRS-1 protein. Conversely, knockdown of FL-Irs-1 mRNA increased Rb mRNA expression and enhanced myoblast differentiation into myotubes.
Conclusions: Our findings suggest that the FL-Irs-1 transcript regulates myogenic differentiation as a regulatory RNA in myoblasts.
Journal Title
BMC Cell Biology
ISSN
26618850
NCID
AA12034785
Publisher
Springer Nature|BioMed Central
Volume
16
Start Page
8
Published Date
2015-03-11
Rights
© 2015 Nagano et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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language
eng
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departments
Medical Sciences