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ID 111119
Title Alternative
DAPI染色による腎アミロイドーシスの新しい鑑別方法
Diagnosis of renal AL amyloidosis using DAPI
Author
Minakuchi, Jun Kawashima Hospital
Keywords
Amyloidosis
DAPI
Renal amyloidosis
AL amyloidosis
AA amyloidosis
Content Type
Thesis or Dissertation
Description
Amyloidosis is often overlooked because its clinical manifestations can mimic those of more-common diseases. It is important to get a precise diagnosis as early as possible for the prevention of further organ damages. Amyloidosis is a disorder caused by deposition of insoluble abnormal amyloid. The kidney is a frequent site of amyloid deposition. The amyloid fibrils have a characteristic appearance and generate birefringence under polarized light when stained with the Congo red dye. Classification of amyloidosis is based on the precursor protein that forms the amyloid fibrils and the distribution of amyloid deposits as either systemic or localized. Involvement of amyloid fibrils in kidneys mainly occurs as amyloid light-chain (AL) or amyloid A (AA) amyloidosis. The potassium permanganate method with Congo red staining was once used widely to discriminate AL and AA amyloidoses, but thismethod has a problem of false positive results. We found that extracellular and cytoplasmic glomerular 4’,6-diamidino-2-phenylindole (DAPI)-positive areas were clearly consistent with amyloid deposition in AL amyloidosis. In contrast, the overlapping staining was not seen in AA amyloidosis. Therefore, we propose that DAPI staining readily distinguishes AL renal amyloidosis from AA renal amyloidosis as a simple and reproducible histochemical method.
Journal Title
The Journal of Medical Investigation
ISSN
13496867
13431420
NCID
AA11166929
AA12022913
Publisher
Faculty of Medicine Tokushima University
Volume
64
Issue
3-4
Start Page
217
End Page
221
Sort Key
217
Published Date
2017-08
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Motokazu Matsuuraの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
乙第2099号
Diploma Number
乙医第1753号
Granted Date
2017-11-30
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
University Hospital
Medical Sciences