ID 113324
Title Alternative
子宮内膜症モデルマウスにおいてLPSは腹膜子宮内膜症初期病巣形成を促進する
LPS proliferate the endometriosis
Author
Kadota, Yuri Tokushima University
Erdenebayar, Otgontsetseg Tokushima University
Kasai, Kana Tokushima University
Tani, Anna Iseikai Hospital
Matsui, Sumika Tokushima Red Cross Hospital KAKEN Search Researchers
Maegawa, Masahiko Tokushima Prefectural Central Hospital
Keywords
LPS
endometriosis
inflammation
Murine model
cytokine
mice model
Content Type
Thesis or Dissertation
Description
Purpose : The aims of this study were to clarify the effects of lipopolysaccharide (LPS) on the early development of endometriosis and on the production of cytokines and chemokines in the murine peritoneal cavity. Methods : Endometriotic lesions were induced in C57BL/6J adult female mice by intraperitoneal injection of endometrial fragments plus blood or endometrial fragments plus blood with LPS. On day 7, endometriotic lesions were assessed by gross and microscopic evaluations. Time-dependent changes in the secretion of TNF-α, IL-6, and CXCL2/MIP-2 in peritoneal lavage fluid after the intraperitoneal injection of LPS (50 µg/body) were measured by their respective enzyme-linked immunosorbent assays. Results : The areas of endometriotic lesions in the LPS group (10.8±8.6 mm2) were significantly larger than those in the control group (3.1±3.7 mm2). The levels of TNF-α and IL-6 peaked within 2 hours and the level of MIP-2 reached a maximum on day 1 after the injection of LPS. Conclusions : LPS promotes development of the early stages of murine endometriotic lesions.
Journal Title
The Journal of Medical Investigation
ISSN
13496867
13431420
NCID
AA12022913
AA11166929
Publisher
Tokushima University Faculty of Medicine
Volume
66
Issue
1-2
Start Page
70
End Page
74
Sort Key
70
Published Date
2019-02
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Kaoru Keyamaの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3248号
Diploma Number
甲医第1397号
Granted Date
2018-12-27
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
University Hospital
Medical Sciences