ID | 109655 |
Author |
Hossain, Md. Golam
Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School
Iwata, Takeo
Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School
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Mizusawa, Noriko
Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School
Tokushima University Educator and Researcher Directory
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Shima, Shahidan Wan Nazatul
Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School
Okutsu, Toru
Department of Oral and Maxillofacial Prosthodontics, Institute of Health Biosciences, The University of Tokushima Graduate School
Ishimoto, Kyoko
Department of Orthodontics and Dentofacial Orthopedics, Institute of Health Biosciences, The University of Tokushima Graduate School
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Yoshimoto, Katsuhiko
Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School
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|
Keywords | Compressive force
preadipocytes
adipogenesis
COX-2
PPARγ2
C/EBPα
|
Content Type |
Journal Article
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Description | Various mechanical stimuli affect differentiation of mesoderm-derived cells such as osteoblasts or myoblasts, suggesting that adipogenesis may be also influenced by mechanical stimulation. However, effects of mechanical stimuli on adipogenesis are
scarcely known. Compressive force was applied to a human preadipocyte cell line, SGBS. Levels of gene expression were estimated by real-time reverse transcription-polymerase chain reaction. The accumulation of lipids was evaluated by Sudan III or Oil Red O staining. In SGBS cells subjected to a compressive force of 226 Pa for 12 h before adipogenic induction, adipogenesis was inhibited. Compressive force immediately after adipogenic induction did not affect the adipogenesis. The expression of peroxisome proliferator-activated receptor (PPAR) γ2 and CCAAT/enhancer binding protein (C/EBP) α mRNA during adipogenesis was inhibited by compressive force, whereas C/EBPβ and C/EBPδ mRNA levels were unaffected. In preadipocytes, compressive force increased mRNA levels of Krüppel-like factor 2, preadipocyte factor 1, WNT10b, and cyclooxygenase-2 (COX-2) which are known as negative regulators for the PPARγ2 and C/EBPα genes. Furthermore, a COX-2 inhibitor completely reversed the inhibition of adipogenesis by compressive force. In conclusion, compressive force inhibited adipogenesis by suppressing expression of PPARγ2 and C/EBPα in a COX-2-dependent manner. |
Journal Title |
Journal of Bioscience and Bioengineering
|
ISSN | 13891723
|
NCID | AA11307678
|
Volume | 109
|
Issue | 3
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Start Page | 297
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End Page | 303
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Sort Key | 297
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Published Date | 2010-03
|
Remark | © 2009, The Society for Biotechnology, Japan. All rights reserved.
© 2009. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
Author
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departments |
Oral Sciences
University Hospital
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