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ID 105888
Title Alternative
脂肪由来幹細胞の栄養因子を介した肝傷害に対しての保護効果
Author
Saito, Yu Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Utsunomiya, Tohru The University of Tokushima KAKEN Search Researchers
Sugimoto, Kouji The University of Tokushima
Asanoma, Michihito The University of Tokushima
Keywords
Adipose derived stem cells
Hepatocytes
Vascular endothelial growth factor
Content Type
Thesis or Dissertation
Description
Background
In this study we investigated whether adipose-derived stem cells (ADSCs) had any beneficial protective effects on liver injury and regeneration in vivo. Moreover, we examined whether ADSCs protect hepatocytes by trophic molecules.
Materials and Methods
We transplanted ADSCs into mice after 70% hepatectomy (Hx) and ischemia-reperfusion (I/R), and observed liver injury and regeneration after reperfusion. We co-cultured hepatocytes with ADSCs using a Transwell System for seven days and evaluated the viabilities of hepatocytes and the cytokine levels in the culture medium. Bevacizumab was used in order to confirm the effect of VEGF on hepatocytes.
Results
ADSCs improved serum liver function at six hours after reperfusion in a non-lethal model and stimulated liver regeneration at 24 hours after reperfusion in a lethal model. VEGF levels in the culture medium increased by co-culture ADSCs with hepatocytes. ADSCs improved the viabilities of hepatocytes. The inhibited production of VEGF by bevacizumab did not affect the viability of hepatocytes.
Conclusions
ADSCs were able to ameliorate liver injury and stimulate liver regeneration in subsequent Hx and I/R injured model mice. Furthermore, hepatocytes were protected by the trophic molecules of the ADSCs. However, such protective effects might be provided by mechanisms other than VEGF signaling.
Journal Title
Journal of Surgical Research
ISSN
00224804
NCID
AA00708021
Publisher
Elsevier
Volume
180
Issue
1
Start Page
162
End Page
168
Published Date
2012-10-25
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨 : LID201308190003.pdf
論文本文 : LID201405260001.pdf
本論文は, 著者Yu Saitoの学位論文として提出され, 学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第2618号
Diploma Number
甲医第1163号
Granted Date
2013-05-23
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
Medical Sciences
University Hospital