Streptococcus intermedius バイオフィルム形成に及ぼす細胞外DNA及びDNA結合タンパク質の影響
Effect of eDNA on biofilm formation
Asikin, Nur The University of Tokushima
Hirota, Katsuhiko The University of Tokushima KAKEN Search Researchers
Yumoto, Hiromichi The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Hirao, Kouji The University of Tokushima KAKEN Search Researchers
Liu, Dali Shanghai Jiao Tong University
Takahashi, Kanako The University of Tokushima KAKEN Search Researchers
Murakami, Keiji The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Matsuo, Takashi The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Shu, Rong Shanghai Jiao Tong University
Miyake, Yoichiro The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Thesis or Dissertation
Aims: The aim of this study was to clarify the effects of homologous and heterologous extracellular DNAs (eDNAs) and histone-like DNA binding protein (HLP) on Streptococcus intermedius biofilm development and rigidity.
Methods and Results: Formed biofilm mass was measured with 0.1% crystal violet staining method and observed with a scanning electron microscope. The localizations of eDNA and extracellular HLP (eHLP) in formed biofilm were detected by staining with 7-hydoxyl-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) and anti-HLP antibody without fixation, respectively. DNase I treatment (200 U ml-1) markedly decreased biofilm formation and cell density in biofilms. Co-localization of eHLP and eDNA in biofilm was confirmed. The addition of eDNA (up to 1 μg ml-1) purified from S. intermedius, other Gram-positive, –negative bacteria, or human KB cells into the S. intermedius culture increased the biofilm mass of all tested strains of S. intermedius, wild-type, HLP down-regulated strain, and control strains. In contrast, the addition of eDNA (> 1 μg ml-1) decreased the biofilm mass of all S. intermedius strains.
Conclusions: These findings demonstrated that eDNA and eHLP play crucial roles in biofilm development and its rigidity.
Significance and Impact of the Study: eDNA- and HLP-targeting strategies may be applicable to novel treatments for bacterial biofilm-related infectious diseases.
Journal of Applied Microbiology
The Society for Applied Microbiology|John Wiley & Sons Inc
内容要旨 : LID201409191007.pdf
審査要旨 : LID201409191008.pdf
論文本文 : k2757_fulltext.pdf
This is the peer reviewed version of the following article: Nur, A., Hirota, K., Yumoto, H., Hirao, K., Liu, D., Takahashi, K., Murakami, K., Matsuo, T., Shu, R. and Miyake, Y. (2013), Effects of extracellular DNA and DNA‐binding protein on the development of a Streptococcus intermedius biofilm. J Appl Microbiol, 115: 260-270., which has been published in final form at https://doi.org/10.1111/jam.12202. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
|DOI (Published Version)|
|URL ( Publisher's Version )|
LID201409191007.pdf 333 KB
LID201409191008.pdf 420 KB
k2757_fulltext.pdf 1.2 MB
|MEXT report number||
Doctor of Dental Science