ID 111178
Title Alternative
マウス肺気腫モデルにおける胎仔肺移植による肺再生
Fetal tissue for mouse lung regeneration
Author
Uyama, Koh The University of Tokushima
Sakiyama, Shoji The University of Tokushima KAKEN Search Researchers
Kenzaki, Koichiro The University of Tokushima
Okumura, Kazumasa The University of Tokushima KAKEN Search Researchers
Keywords
Pallid mouse
COPD
lung regeneration
fetal tissue
pulmonary emphysema
Content Type
Thesis or Dissertation
Description
The mortality and morbidity of chronic obstructive pulmonary disease are high. However, no radical therapy has been developed to date. The purpose of this study was to evaluate whether fetal mouse lung tissue can grow and differentiate in the emphysematous lung. Fetal lung tissue from green fluorescent protein C57BL/6 mice at 16 days’ gestation was used as donor material. Twelve-month-old pallid mice were used as recipients. Donor lungs were cut into small pieces and implanted into the recipient left lung by performing thoracotomy under anesthesia. The recipient mice were sacrificed at day 7, 14, and 28 after implantation and used for histological examination. Well-developed spontaneous pulmonary emphysema was seen in 12-month-old pallid mice. Smooth and continuous connection between implanted fetal lung tissue and recipient lung was recognized. Air space expansion and donor tissue differentiation were observed over time. We could clearly distinguish the border zones between injected tissue and native tissue by the green fluorescence of grafts. Fetal mouse lung fragments survived and differentiated in the emphysematous lung of pallid mice. Implantation of fetal lung tissue in pallid mice might lead to further lung regeneration research from the perspective of respiratory and exercise function.
Journal Title
The Journal of Medical Investigation
ISSN
13496867
13431420
NCID
AA11166929
AA12022913
Publisher
Faculty of Medicine Tokushima University
Volume
63
Issue
3-4
Start Page
182
End Page
186
Sort Key
182
Published Date
2016-08
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨 : LID201610171001.pdf
論文本文 : LID201610171002.pdf
本論文は,著者Koh Uyamaの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第2963号
Diploma Number
甲医第1294号
Granted Date
2016-07-28
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
Medical Sciences
University Hospital