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ID 114676
Title Alternative
Cystic Fibrosis Transmembrane Conductance Regulatorは微小管依存的なカンピロバクター・ジェジュニの侵入を抑制する
Role of CFTR in Campylobacter jejuni infection
Author
Kido, Junko Tokushima University
Amano, Sachie Tokushima University
Hatayama, Sho Tokushima University
Nguyen, Quoc Anh Tokushima University
Sato, Yuri Tokushima University
Kanda, Yuna Tokushima University
Tentaku, Aya Tokushima University
Fukushima, Shiho Tokushima University
Keywords
CFTR
Campylobacter jejuni
cell invasion
host response to infection
intracellular bacteria
microtubule
Content Type
Thesis or Dissertation
Description
Campylobacter jejuni (C. jejuni) is gastroenteritis inducible food-born pathogen. Invasion and adhesion process are essential for leading gastroenteritis in C. jejuni infection process. As against bacterial strategy for efficacy invasion and adhesion, mucosal layer play a key role in defense systems, which modulated by several ion channels and transporters mediated water flux on the intestine. Cystic fibrosis transmembrane conductance regulator (CFTR) play the main role in waterfulux in intestine, and it closely related with bacterial clearance. We previously reported that C. jejuni infection suppresses CFTR channel activity in intestinal epithelial cells, however the mechanism and importance of this suppression is unclear. This study seeks to elucidate the role of CFTR in C. jejuni-infection. Using HEK293 cells that stably express wild type and mutated CFTR, we found that CFTR attenuated C. jejuni invasion, it was not involved bacterial adhesion or intracellular survival but associated with microtubule-dependent cellular transport. Moreover we revealed that CFTR attenuated function of microtubule motor protein but not microtubule stability, which causes inhibition of C. jejuni-invasion. Meanwhile, the CFTR mutant G551D-CFTR, which has defects in channel activity, suppressed C. jejuni-invasion, whereasΔF508-CFTR, which has defects in maturation, did not suppress, suggesting that CFTR suppression of C. jejuni-invasion is related to CFTR maturation but not channel activity.
Taken together, mature CFTR inhibited C. jejuni invasion by regulating microtubule-mediated pathways. We suggest that CFTR plays a critical role in cellular defenses against C. jejuni-invasion, and CFTR suppression may be an initial step in promoting cellular invasion during C. jejuni-infection.
Journal Title
Infection and Immunity
ISSN
10985522
NCID
AA12050872
AA1200309X
Publisher
American Society for Microbiology
Volume
85
Issue
10
Start Page
e00311-17
Published Date
2017-09-20
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Junko Kidoの学位論文として提出され,学位審査・授与の対象となっている。
Rights
© 2017 American Society for Microbiology
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3409号
Diploma Number
甲栄第270号
Granted Date
2020-03-23
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University
departments
Medical Sciences
Bioscience and Bioindustry