number of access : ?
number of downloads : ?
ID 110120
Title Alternative
過剰負荷を受けた顎関節におけるヒアルロン酸代謝の検討
Hyaluronan metabolism in overloaded TMJ
Author
Shinohara, Takehiro Tokushima University
Mino-Oka, Akiko Tokushima University
Inubushi, Toshihiro Sanford Burnham Medical Research Institute
Yamaguchi, Y Sanford Burnham Medical Research Institute
Keywords
Hyaluronan
temporomandibular joint
osteoarthritis
Content Type
Thesis or Dissertation
Description
The present study aimed to examine hyaluronan (HA) metabolism in relation to the onset and progression of temporomandibular joint osteoarthritis (TMJ-OA) induced by mechanical overloading. Two-month-old and 6-month-old C57BL/6N mice were divided into experimental and untreated control groups (n = 5/group). A sliding plate was attached to the maxillary incisors of the experimental mice for 10 days to overload the condylar cartilage in TMJ. In experimental group, profound cartilage degradation was detected in hematoxylin-eosin, Safranin-O-Fast Green stained sections. It was also shown that the cartilage degradation was greater in older mice in both the control and the experimental groups. The number of HABP positive cells was decreased by mechanical overloading and with age. The reduction of HA expression was correlated with the progression of cartilage degradation induced by mechanical overloading. The absolute quantification of the mRNA expression related to HA synthesis and HA degradation was also performed in each group. The mRNA expression levels of HA synthase (HAS) 2 and 3 were lower in the experimental group compared with the control group in the younger mice. In contrast, the mRNA expression levels of the HA degradation gene, HYAL2 and KIAA1199, were higher in the experimental group compared with the control group in the older mice. Thus, mechanical overload differently affected the balance of HA degradation and HA synthesis in the older and younger mice, respectively. In conclusion, mechanical overloading affects HA metabolism and it might initiate or amplify the condylar cartilage degradation.
Journal Title
Journal of Oral Rehabilitation
ISSN
0305182X
13652842
NCID
AA00704042
AA11628445
Publisher
John Wiley & Sons
Volume
43
Issue
12
Start Page
921
End Page
928
Published Date
2016-09-14
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨:LID201704281007.pdf
審査要旨:LID201704281008.pdf
論文本文:k3073_fulltext.pdf
著者の申請により要約(2017-04-28公開)に替えて論文全文を公開(2019-06-13)
本論文は, 著者T. Shinoharaの学位論文として提出され, 学位審査・授与の対象となっている。
Rights
This is the peer reviewed version of the following article: Shinohara, T. , Izawa, T. , Mino‐Oka, A. , Mori, H. , Iwasa, A. , Inubushi, T. , Yamaguchi, Y. and Tanaka, E. (2016), Hyaluronan metabolism in overloaded temporomandibular joint. J Oral Rehabil, 43: 921-928. doi:10.1111/joor.12443, which has been published in final form at https://doi.org/10.1111/joor.12443. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3073号
Diploma Number
甲口第416号
Granted Date
2017-03-23
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
Oral Sciences