Total for the last 12 months
number of access : ?
number of downloads : ?
ID 115825
Title Alternative
Molecular analysis for bacterial contamination
Author
Kokeguchi, Susumu Okayama University
Keywords
Bacterial contamination
Dental unit water line
Molecular analysis
PCR-DGGE
Content Type
Journal Article
Description
Bacterial contamination in dental unit waterlines (DUWLs) was evaluated by molecular techniques in addition to the conventional culture method. Water samples (n=8) from DUWLs were investigated for heterotrophic bacteria by culture method using R2A agar. The selected bacterial antibiotic-resistance genes and Legionella species-specific 16SrDNA were identified by PCR. The profiles of bacterial contamination in DUWLs were further identified by PCR-DGGE. In this study, no antibiotic-resistant or Legionella genes were detected. Polycyclic aromatic hydrocarbon-degrading bacterium, Novosphingobium sp. was the most prevalent in DUWLs. Conventional PCR and PCR-DGGE were shown to be potentially useful for monitoring of bacterial contamination in DUWLs.
Journal Title
New Microbiologica
ISSN
11217138
NCID
AA11980981
Publisher
EDIMES
Volume
39
Issue
2
Start Page
143
End Page
145
Published Date
2016-04
EDB ID
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Oral Sciences