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ID 114285
Title Alternative
乳児期早期における鶏卵、牛乳アレルゲン特異的免疫グロブリンのクラススイッチを伴うアイソタイプ形成経過と、湿疹による低親和性、高親和性の抗原特異的IgE抗体の形成
Author
Shinahara, Wakako Tokushima University
Ogawa, Yukiko Tokushima Prefecture Naruto Hospital
Shitsukawa, Keiji Tokushima Prefecture Naruto Hospital
Kubota, Kenji Tokushima University
Yang, Limin National Center for Child Health and Development
Ohya, Yukihiro National Center for Child Health and Development
Saito, Hirohisa National Center for Child Health and Development
Keywords
allergen microarray
eczema
food allergy
high‐affinity immunoglobulin E
immunoglobulin isotype formation
low‐affinity immunoglobulin E
Content Type
Thesis or Dissertation
Description
Introduction: Allergen‐specific immunoglobulin isotype formation associated with immunoglobulin class‐switching during the lactation period is the immunological background for food allergy in infants. We analyzed the serial changes in the production of feeding type‐related egg‐ and milk‐specific immunoglobulin isotypes from birth to 6 months of age with or without eczema in 84 infants.
Methods: Allergen‐specific immunoglobulin G1 (IgG1), IgG2, IgG3, IgG4, IgA, and IgE levels of hen’s egg and bovine milk were measured in cord blood and blood samples from infants at 2, 4, and 6 months of age by the densely carboxylated protein microarray.
Results: Formula and mixed feeding were associated with a rapid increase in cow’s milk allergen‐specific immunoglobulins and feeding type‐related significant differences in casein‐specific immunoglobulin levels were detected. Breast and mixed feeding were associated with slow but significant increase in ovalbumin‐specific IgG1 and IgE levels, but not other immunoglobulins. We found two different immunoglobulin isotype formation at 6 months of age with low‐ or high‐affinity IgE against ovalbumin. One isotype formation pattern had relatively high ovalbumin‐specific IgG1 levels, detectable IgG2, and low‐affinity IgE, while the other had low ovalbumin‐specific IgG1 levels, undetectable IgG2, and high levels of high‐affinity IgE. The incidence of eczema was significantly higher in the latter pattern (84.6%), compared with the remaining infants (42.2%).
Conclusions: Feeding practice‐related allergen sensitization and immunoglobulin isotype formation were identified during the lactation period. The development of eczema during the lactation period could potentially modify the immunoglobulin isotype formation with high levels of high‐affinity IgE.
Journal Title
Immunity, Inflammation and Disease
ISSN
20504527
Publisher
Wiley
Volume
7
Issue
2
Start Page
74
End Page
85
Published Date
2019-03-11
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Makoto Iraharaの学位論文として提出され,学位審査・授与の対象となっている。
Rights
This is an open access article under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/4.0/ ), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3345号
Diploma Number
甲医第1435号
Granted Date
2020-01-23
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
University Hospital
Medical Sciences
Institute of Advanced Medical Sciences