ID 112975
Title Alternative
幼若雌ラットにおける発育に伴う卵巣Kiss1,Kiss1r遺伝子発現の変動およびHCGに対する感受性
OVARIAN Kiss1 mRNA IN THE PREPUBERTAL PERIOD
Author
Taniguchi, Yuka Tokushima University
Yano, Yuya Tokushima University
Keywords
Development
kisspeptin
Kiss1
Kiss1r
Ovary
Content Type
Thesis or Dissertation
Description
Kisspeptin, which is encoded by the Kiss1 gene, and its receptor, the G protein-coupled receptor 54 (Kiss1r), play important roles in the regulation of reproductive functions in mammals. Several studies have shown that the Kiss1 and Kiss1r genes are expressed in the rat, primate, and human ovaries, and that the ovarian kisspeptin system plays a pivotal role in ovulation at the proestrous stage in adulthood. The purpose of this study was to evaluate development-related changes in the expression of ovarian Kiss1 and Kiss1r genes and in kisspeptin levels, and to identify the regulatory factors for these genes during the prepubertal period. The serum kisspeptin level was also measured to examine whether ovarian kisspeptin affects serum kisspeptin levels. Variations in the ovarian Kiss1 and Kiss1r mRNA levels were observed during the prepubertal period in female rats, with levels peaking around postnatal days 20 and 15, respectively. Nevertheless, the ovarian kisspeptin content per total protein level was stably maintained. Serum kisspeptin levels at postnatal days 30 and 35 were higher than those at earlier postnatal days. The pattern of the ovarian Kiss1 mRNA levels was similar to that of the serum luteinizing hormone (LH) levels, and the ovarian Kiss1 mRNA level increased after injection with human chorionic gonadotropin (HCG) on postnatal day 20, but not on postnatal days 10 and 30. These data indicate that ovarian Kiss1 and Kiss1r mRNA levels are increased on postnatal days 20 and 15, respectively, and that changes in the serum LH level and the ovarian sensitivity to LH may be involved in the alteration of ovarian Kiss1 mRNA levels.
Journal Title
Journal of Reproduction and Development
ISSN
13484400
09168818
NCID
AA10936678
Publisher
Society for Reproduction and Development
Volume
63
Issue
4
Start Page
409
End Page
414
Published Date
2017-05-27
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Mikio YAMASAKIの学位論文として提出され,学位審査・授与の対象となっている。
Rights
©2017 Society for Reproduction and Development This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3105号
Diploma Number
甲医第1349号
Granted Date
2017-09-28
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
University Hospital
Medical Sciences