ID | 115732 |
Author |
Masaki, Kaito
Shinshu University
Sakai, Mizuki
Shinshu University
Jo, Jun-Ichiro
Kyoto University
Hoshina, Kazuo
Nagano Animal Industry Experiment Station
Fujimori, Yuki
Nagano Animal Industry Experiment Station
Oka, Kenji
Shinshu University
Amano, Toshiyasu
Nagano Red Cross Hospital
Yamanaka, Takahiro
Shinshu University
Tabata, Yasuhiko
Kyoto University
Shiozawa, Tanri
Shinshu University
Ishizuka, Osamu
Shinshu University
Hochi, Shinichi
Shinshu University
Takashima, Seiji
Shinshu University
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Content Type |
Journal Article
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Description | Both glial cell line-derived neurotrophic factor (GDNF) and fibroblast growth factor 2 (FGF2) are bona fide self-renewal factors for spermatogonial stem cells, whereas retinoic acid (RA) induces spermatogonial differentiation. In this study, we investigated the functional differences between FGF2 and GDNF in the germline niche by providing these factors using a drug delivery system in vivo. Although both factors expanded the GFRA1+ subset of undifferentiated spermatogonia, the FGF2-expanded subset expressed RARG, which is indispensable for proper differentiation, 1.9-fold more frequently than the GDNF-expanded subset, demonstrating that FGF2 expands a differentiation-prone subset in the testis. Moreover, FGF2 acted on the germline niche to suppress RA metabolism and GDNF production, suggesting that FGF2 modifies germline niche functions to be more appropriate for spermatogonial differentiation. These results suggest that FGF2 contributes to induction of differentiation rather than maintenance of undifferentiated spermatogonia, indicating reconsideration of the role of FGF2 in the germline niche.
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Journal Title |
Stem Cell Reports
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ISSN | 22136711
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Publisher | International Society for Stem Cell Research|Cell Press
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Volume | 10
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Issue | 6
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Start Page | 1782
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End Page | 1792
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Published Date | 2018-04-19
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Rights | This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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DOI (Published Version) | |
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language |
eng
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TextVersion |
Publisher
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departments |
Institute of Advanced Medical Sciences
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