Ushigoe, Kenjiro The University of Tokushima
Yano, Yuya The University of Tokushima
Taniguchi, Yuka The University of Tokushima
Yamamoto, Yuri The University of Tokushima Tokushima University Educator and Researcher Directory
Yasui, Toshiyuki The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Irahara, Minoru The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
tumor necrosis factor alpha
Thesis or Dissertation
Aim: Cytokine-induced neutrophil chemoattractant (CINC/gro) is a CXC family chemokine, similar to interleukin-8 in rats, and is one of the factors that regulates ovulation. However, the mechanism that regulates atresia of the ovaries postovulation is not clearly defined.
Methods: Whether antibody-blocking of CINC/gro can alter the number of ovulated oocytes and modulate neutrophil infiltration was investigated. The effect of the antibody on the level of inflammatory cytokine production and follicular atresia was examined. Apoptosis was measured by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method and via analysis of the messenger RNA expression of Bcl-2 and Bcl2-associated X (Bax).
Results: The anti-CINC/gro antibody treatment decreased the number of ovulated oocytes. The messenger RNA levels of cyclooxygenase-2 and interleukin-1 beta were decreased by the antibody treatment, whereas that of tumor necrosis factor (TNF) alpha was increased. The TUNEL analysis revealed a larger number of apoptotic cells in the antibody group, compared with those in the control group, as well as a significant increase in the Bax/Bcl-2 ratio 24 hours after human chorionic gonadotropin administration.
Conclusion: These findings suggest that ovulation is accelerated by neutrophil infiltration into the theca layer. The CINC/gro appears to synergize with interleukin-1 beta for ovulation. By contrast, the data suggest that CINC/gro expression suppresses TNF alpha expression and that CINC/gro expression therefore prevents the follicles from undergoing atresia and apoptosis.
Reproductive Medicine and Biology
Wiley|Japan Society for Reproductive Medicine
本論文は, 著者Yu Tanakaの学位論文として提出され, 学位審査・授与の対象となっている。
This is an open access article under the terms of the Creative Commons Attribution License(https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
© 2017 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine.
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|MEXT report number
Doctor of Medical Science