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ID 116220
Title Alternative
Role of vinculin in paracellular barrier for ions
Author
Konishi, Satoshi Osaka University|Kyoto University
Yano, Tomoki Osaka University
Tanaka, Hiroo Osaka University|Teikyo University
Mizuno, Tomoaki Osaka University
Kanoh, Hatsuho Osaka University|Kyoto University
Tsukita, Kazuto Osaka University|Kyoto University
Namba, Toshinori Tokyo University
Tamura, Atsushi Osaka University|Teikyo University
Gotoh, Shimpei Kyoto University
Matsumoto, Hisako Kyoto University
Hirai, Toyohiro Kyoto University
Tsukita, Sachiko Osaka University|Teikyo University
Content Type
Journal Article
Description
The paracellular barrier function of tight junctions (TJs) in epithelial cell sheets is robustly maintained against mechanical fluctuations, by molecular mechanisms that are poorly understood. Vinculin is an adaptor of a mechanosensory complex at the adherens junction. Here, we generated vinculin KO Eph4 epithelial cells and analyzed their confluent cell-sheet properties. We found that vinculin is dispensable for the basic TJ structural integrity and the paracellular barrier function for larger solutes. However, vinculin is indispensable for the paracellular barrier function for ions. In addition, TJs stochastically showed dynamically distorted patterns in vinculin KO cell sheets. These KO phenotypes were rescued by transfecting full-length vinculin and by relaxing the actomyosin tension with blebbistatin, a myosin II ATPase activity inhibitor. Our findings indicate that vinculin resists mechanical fluctuations to maintain the TJ paracellular barrier function for ions in epithelial cell sheets.
Journal Title
Life Science Alliance
ISSN
25751077
Publisher
Life Science Alliance
Volume
2
Issue
4
Start Page
e201900414
Published Date
2019-08-09
Rights
This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
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DOI (Published Version)
URL ( Publisher's Version )
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language
eng
TextVersion
Publisher
departments
Medical Sciences