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ID 114230
Author
Iwasaki, Sota Tokushima University
Becchaku, Yuta Tokushima University
Yabe, Shun Toho University
Morita, Kotaro Toho University
Hirayama, Naoki Toho University
Keywords
Carbon nanodot
characterization
capillary electrophoresis
micellar electrokinetic chromatography
affinity capillary electrophoresis
Content Type
Journal Article
Description
Water-soluble carbon nanodots (CND) were synthesized under microwave irradiation from glutamic acid or glutamic acid–boric acid mixture. The CNDs were collected in an aqueous solution through size fractionation by centrifugal filtration. The CNDs thus prepared were subjected to characterization by capillary electrophoresis (CE). A peak signal of anionic substance was detected in the electropherogram, and it was found to be a major component of the CNDs. The effective electrophoretic mobility of the major component was almost identical over the pH range between 6.7 and 11.6, suggesting that the functional group of amine or boric acid moiety was not included in the CNDs. The effective electrophoretic mobility decreased at an acidic pH of less than 5, and it was suggested that carboxylate moiety was included in the CNDs. A signal of less-charged CNDs was also detected in the electropherogram, and the CNDs were characterized by a CE format of micellar electrokinetic chromatography. Two or four peaks were detected just after the electroosmotic flow; the less-charged CNDs were thus hydrophilic. The affinity interaction was also examined between the major anionic CNDs and a hydrophobic pairing cation. The peak signal of the major anionic CNDs broadened, and its theoretical number of plates decreased in the presence of tetrabutylammonium ion in the separation buffer. A small portion of the anionic CNDs were a little hydrophobic at different degrees, and their effective electrophoretic mobility decreased by the hydrophobic interaction, resulting in peak broadening of the anionic CNDs.
Journal Title
Analytical Sciences
ISSN
13482246
09106340
NCID
AA10500785
Publisher
The Japan Society for Analytical Chemistry
Volume
36
Issue
8
Start Page
941
End Page
946
Published Date
2020-08-10
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Science and Technology