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ID 114213
Title Alternative
骨-歯根膜線維の複合組織形成による三次元的な歯周組織再生技術の開発
Author
Raju, Resmi Tokushima University
Huijiao, Yan Tokushima University
Waskitho, Arief Tokushima University
Inoue, Masahisa Tokushima Bunri University
Keywords
Cell sheet
Periodontal tissue
Regeneration
Periodontal ligament cell
Content Type
Thesis or Dissertation
Description
Periodontal tissue is a distinctive tissue structure composed three-dimensionally of cementum, periodontal ligament (PDL) and alveolar bone. Severe periodontal diseases cause fundamental problems for oral function and general health, and conventional dental treatments are insufficient for healing to healthy periodontal tissue. Cell sheet technology has been used in many tissue regenerations, including periodontal tissue, to transplant appropriate stem/progenitor cells for tissue regeneration of a target site as a uniform tissue. However, it is still difficult to construct a three-dimensional structure of complex tissue composed of multiple types of cells, and the transplantation of a single cell sheet cannot sufficiently regenerate a large-scale tissue injury. Here, we fabricated a three-dimensional complex cell sheet composed of a bone-ligament structure by layering PDL cells and osteoblast-like cells on a temperature responsive culture dish. Following ectopic and orthotopic transplantation, only the complex cell sheet group was demonstrated to anatomically regenerate the bone-ligament structure along with the functional connection of PDL-like fibers to the tooth root and alveolar bone. This study represents successful three-dimensional tissue regeneration of a large-scale tissue injury using a bioengineered tissue designed to simulate the anatomical structure.
Journal Title
Scientific Reports
ISSN
20452322
Publisher
Springer Nature
Volume
10
Start Page
1656
Published Date
2020-02-03
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨 : k3381_abstract.pdf
審査要旨 : k3381_review.pdf
論文本文 : k3381_fulltext.pdf (著者最終稿)
論文本文 : srep_10_1656.pdf (雑誌掲載版)
本論文は, 著者Resmi Rajuの学位論文として提出され, 学位審査・授与の対象となっている。
Rights
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3381号
Diploma Number
甲口第460号
Granted Date
2020-03-23
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
Oral Sciences