ID | 117991 |
Author |
Mizuguchi, Hitoshi
Tokushima University
Tokushima University Educator and Researcher Directory
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Fujiki, Soma
Tokushima University
Shibata, Takaki
Tokushima University
Iiyama, Masamitsu
Nomura Micro Science
Takayanagi, Toshio
Tokushima University
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Lin, Yu-Chi
National Taiwan University of Science and Technology
Yeh, Min-Hsin
National Taiwan University of Science and Technology
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Keywords | Track-etched membrane electrode
Enzyme-free electrochemical biosensor
Uric acid
Acetylene black
2-aminoethanethiol
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Content Type |
Journal Article
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Description | A flow-based, enzyme-free biosensor for the selective and sensitive determination of uric acid was developed using track-etched membrane electrodes (TEMEs). The proposed sensor system comprises a pre-reactor electrode to decompose interfering species, such as L-ascorbic acid, and a detector electrode to detect uric acid. TEMEs were prepared by sputter deposition of gold on a track-etched membrane filter. Acetylene black (AB) was introduced as an electrode catalyst by simple filtration with the TEME, and the sensitivity for uric acid using the AB-decorated Au-TEME was much greater than that with bare Au-TEME. The insertion of the 2-aminoethanethiol-modified Au-TEMEs as pre-reactor electrodes upstream of the AB-decorated detector electrode eliminated the interference caused by the co-existence of L-ascorbic acid because the interfering species were electrolyzed at the pre-reactor electrode prior to detection of uric acid. The proposed sensor system was incorporated into a flow-injection analysis system to evaluate the sensor performance. The detection limit of uric acid was 0.6 μM. A recovery test by standard addition to human urine samples also showed satisfactory results.
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Journal Title |
Sensors and Actuators B : Chemical
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ISSN | 09254005
18733077
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NCID | AA1078104X
AA11540366
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Publisher | Elsevier
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Volume | 383
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Start Page | 133588
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Published Date | 2023-02-26
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Remark | 論文本文は2025-02-26以降公開予定
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Rights | © 2023. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
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EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
language |
eng
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TextVersion |
その他
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departments |
Science and Technology
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