Total for the last 12 months
number of access : ?
number of downloads : ?
ID 117991
Author
Fujiki, Soma Tokushima University
Shibata, Takaki Tokushima University
Iiyama, Masamitsu Nomura Micro Science
Lin, Yu-Chi National Taiwan University of Science and Technology
Yeh, Min-Hsin National Taiwan University of Science and Technology
Keywords
Track-etched membrane electrode
Enzyme-free electrochemical biosensor
Uric acid
Acetylene black
2-aminoethanethiol
Content Type
Journal Article
Description
A flow-based, enzyme-free biosensor for the selective and sensitive determination of uric acid was developed using track-etched membrane electrodes (TEMEs). The proposed sensor system comprises a pre-reactor electrode to decompose interfering species, such as L-ascorbic acid, and a detector electrode to detect uric acid. TEMEs were prepared by sputter deposition of gold on a track-etched membrane filter. Acetylene black (AB) was introduced as an electrode catalyst by simple filtration with the TEME, and the sensitivity for uric acid using the AB-decorated Au-TEME was much greater than that with bare Au-TEME. The insertion of the 2-aminoethanethiol-modified Au-TEMEs as pre-reactor electrodes upstream of the AB-decorated detector electrode eliminated the interference caused by the co-existence of L-ascorbic acid because the interfering species were electrolyzed at the pre-reactor electrode prior to detection of uric acid. The proposed sensor system was incorporated into a flow-injection analysis system to evaluate the sensor performance. The detection limit of uric acid was 0.6 μM. A recovery test by standard addition to human urine samples also showed satisfactory results.
Journal Title
Sensors and Actuators B : Chemical
ISSN
09254005
18733077
NCID
AA1078104X
AA11540366
Publisher
Elsevier
Volume
383
Start Page
133588
Published Date
2023-02-26
Remark
論文本文は2025-02-26以降公開予定
Rights
© 2023. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
language
eng
TextVersion
その他
departments
Science and Technology