ID 109479
Title Alternative
MC3T3-E1細胞における圧縮応力誘導性miR494-3pによる細胞増殖の阻害
FUNCTION OF miR-494-3p IN OSTEOBLASTS
Author
Higaki, Nobuaki Tokushima University
Keywords
Mechanical stimuli
Compressive force
MicroRNA
Osteoblasts
Cell proliferation
Content Type
Thesis or Dissertation
Description
Mechanical stimuli regulate fundamental cell processes such as proliferation, differentiation, and morphogenesis. We attempted to identify microRNA (miRNA) whose expression is changed during compressive treatment in MC3T3-E1, a pre-osteoblastic cell line. Microarray analysis followed by reverse transcription-quantitative polymerase chain reaction revealed that compressive force at 294 Pa for 24 h in MC3T3-E1 cells increased levels of miR-494-3p, miR-146a-5p, miR-210-3p, and miR-1247-3p. Among these miRNAs, miR-494-3p was found to inhibit cell proliferation in MC3T3-E1 cells. Furthermore, cells subjected to compressive force showed slower cell growth compared with control cells. Levels of mRNA for fibroblast growth factor receptor 2 (FGFR2) and Rho-associated coiled-coil kinase 1 (ROCK1), which were predicted to be targets of miR-494-3p, were decreased by compressive force or overexpression of miR-494-3p mimics in MC3T3-E1 cells. Furthermore, binding sites of miR-494-3p within 3'-untranslated regions of Fgfr2 and Rock1 were determined using luciferase reporter assay. In conclusion, compressive force affected expressions of several miRNAs including miR-494-3p in MC3T3-E1 cells. Compressive force might inhibit cell proliferation in osteoblasts by up-regulating miR-494-3p followed by FGFR2 and ROCK1 gene repressions.
Journal Title
Journal of Bioscience and Bioengineering
ISSN
13474421
13891723
NCID
AA11644703
AA11307678
Publisher
The Society for Biotechnology, Japan|Elsevier
Volume
120
Issue
4
Start Page
456
End Page
462
Published Date
2015-03-17
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨 : LID201507171004.pdf
審査要旨 : LID201507171005.pdf
論文本文 : k2858_fulltext.pdf
本論文は,著者Yuki Iwawakiの学位論文として提出され,学位審査・授与の対象となっている。
著者の申請により要約(2015-07-18公開)に替えて論文全文を公開(2019-08-22)
Rights
Copyright © 2015 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
This manuscript version is made available under the CC-BY-NC-ND 4.0 license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第2858号
Diploma Number
甲口第397号
Granted Date
2015-06-11
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
Oral Sciences
University Hospital