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ID 110107
Title Alternative
DNAメチル化は3T3-L1脂肪細胞におけるレプチン発現を抑制する
DNA Methylation Suppresses Leptin Expression in Adipocytes
Author
Tominaga, Ayako Tokushima University
Nakagawa, Kasumi Tokushima University
Nishiguchi, Misa Tokushima University
Sebe, Mayu Tokushima University
Miyatake, Yumiko Tokushima University
Kitamura, Tadahiro Japan Society for the Promotion of Science
Keywords
Adipocyte
Leptin
DNA methylation
3T3-L1
obesity
Content Type
Thesis or Dissertation
Description
Leptin is a key regulator of energy intake and expenditure. This peptide hormone is expressed in mouse white adipose tissue, but hardly expressed in 3T3-L1 adipocytes. Using bisulfite sequencing, we found that CpG islands in the leptin promoter are highly methylated in 3T3-L1cells. 5-azacytidine, an inhibitor of DNA methyltransferase, markedly increased leptin expression as pre-adipocytes matured into adipocytes. Remarkably, leptin expression was stimulated by insulin in adipocytes derived from precursor cells exposed to 5-azacytidine, but suppressed by thiazolidinedione and dexamethasone. In contrast, adipocytes derived from untreated precursor cells were unresponsive to both 5-azacytidine and hormonal stimuli, although lipid accumulation was sufficient to boost leptin expression in the absence of demethylation. Taken together, the results suggest that leptin expression in 3T3-L1 cells requires DNA demethylation prior to adipogenesis, transcriptional activation during adipogenesis, and lipid accumulation after adipogenesis.
Journal Title
PLOS ONE
ISSN
19326203
Publisher
PLOS
Volume
11
Issue
8
Start Page
e0160532
Published Date
2016-08-05
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨:LID201704261022.pdf
論文本文:LID201704261023.pdf
本論文は, 著者Masashi Kurodaの学位論文として提出され, 学位審査・授与の対象となっている。
Rights
This is an open access article distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/4.0/ ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3087号
Diploma Number
甲栄第239号
Granted Date
2017-03-23
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University
departments
Medical Sciences