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ID 116344
Title Alternative
C2C12筋管細胞においてモリンはデキサメタゾン誘導性の酸化ストレスと筋萎縮を抑制する
Author
Anaytulla Tokushima University
Miki, Yukari Tokushima University
Higashitani, Atsushi Tohoku University
Kobayashi, Takeshi Nagoya University
Ohno, Ayako Tokushima University
Keywords
Muscle atrophy
Morin
Mouse C2C12 skeletal myotubes
Reactive oxygen species
Ubiquitin ligases
Content Type
Thesis or Dissertation
Description
Glucocorticoids are the drugs most commonly used to manage inflammatory diseases. However, they are prone to inducing muscle atrophy by increasing muscle proteolysis and decreasing protein synthesis. Various studies have demonstrated that antioxidants can mitigate glucocorticoid-induced skeletal muscle atrophy. Here, we investigated the effect of a potent antioxidative natural flavonoid, morin, on the muscle atrophy and oxidative stress induced by dexamethasone (Dex) using mouse C2C12 skeletal myotubes. Dex (10 μM) enhanced the production of reactive oxygen species (ROS) in C2C12 myotubes via glucocorticoid receptor. Moreover, Dex administration reduced the diameter and expression levels of the myosin heavy chain protein in C2C12 myotubes, together with the upregulation of muscle atrophy-associated ubiquitin ligases, such as muscle atrophy F-box protein 1/atrogin-1, muscle ring finger protein-1, and casitas B-lineage lymphoma proto-oncogene-b. Dex also significantly decreased phosphorylated Foxo3a and increased total Foxo3a expression. Interestingly, Dex-induced ROS accumulation and Foxo3a expression were inhibited by morin (10 μM) pretreatment. Morin also prevented the Dex-induced reduction of myotube thickness, together with muscle protein degradation and suppression of the upregulation of atrophy-associated ubiquitin ligases. In conclusion, our results suggest that morin effectively prevents glucocorticoid-induced muscle atrophy by reducing oxidative stress.
Journal Title
Archives of Biochemistry and Biophysics
ISSN
00039861
NCID
AA00547159
AA11539811
Publisher
Elsevier
Volume
704
Start Page
108873
Published Date
2021-04-10
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Anayt Ullaの学位論文として提出され,学位審査・授与の対象となっている。
著者名"Anayt Ulla"について,論文投稿時に著者姓・名が必須項目だったため,論文PDFでは便宜上 "Anayt Ulla" と表記されているが,実際には姓はなく,本名は "Anaytulla" である。
Rights
© 2021 Elsevier Inc. All rights reserved.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3532号
Diploma Number
甲栄286号
Granted Date
2021-09-02
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University
departments
Medical Sciences
University Hospital