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ID 117031
Title Alternative
口腔上皮細胞に対するJuncus effusus L.水抽出物の抗炎症及び保護効果
Author
Wada, Akihiro Tokushima University
Ishikawa, Yumi Osaka Dental University
Amoh, Takashi Mejiro University College KAKEN Search Researchers
Keywords
Juncus effusus L
Oral epithelial cells
Toll-like receptor
Lipopolysaccharide
Chemokines
Content Type
Thesis or Dissertation
Description
Periodontitis is a chronic inflammatory disease caused by periodontopathogenic bacteria that form biofilms in periodontal pockets. The gingival epithelium acts as the first physical barrier in fighting attacks by periodontopathogenic pathogens, such as the primary etiological agent Porphyromonas gingivalis, and various exogenous chemicals, as well as regulates the local innate immune responses. Therefore, the development of novel oral care products to inhibit inflammatory reactions caused by bacterial infection and protect the gingival epithelium is necessary. Juncus effusus L. has generally been used as an indigenous medicine, such as a diuretic, an antipyretic, and an analgesic, in ancient practice. In this study, we examined the effects of a water extract from J. effusus L. on the inhibition of the inflammatory reaction elicited by bacterial infection and protection of the oral epithelium by chemical irritation. Pretreatment of oral epithelial cells with the water extract from J. effusus L. significantly reduced P. gingivalis or its lipopolysaccharide- (LPS-) mediated production of chemokines (interleukin-8 and C-C-chemokine ligand20) in a concentration-dependent manner with comparable to or greater effects than epigallocatechin gallate and protected oral epithelial cells from injury by chemical irritants, cetylpyridinium chloride, and benzethonium chloride. Moreover, the water extract from J. effusus L. in the presence of antimicrobial agents or antifibrinolytics already used as ingredients in mouthwash could significantly reduce the production of chemokines from P. gingivalis LPS-stimulated oral epithelial cells in a concentration-dependent manner. These findings suggest that the water extract from J. effusus L. is potentially useful for oral care to prevent oral infections, such as periodontal infections, and maintain oral epithelial function.
Journal Title
BioMed Research International
ISSN
23146133
23146141
Publisher
Hindawi
Volume
2022
Start Page
9770899
Published Date
2022-01-04
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Akihiro Wadaの学位論文として提出され,学位審査・授与の対象となっている。
Rights
This is an open access article distributed under the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/4.0/ ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3612号
Diploma Number
甲口第482号
Granted Date
2022-03-23
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
Oral Sciences
University Hospital