Capsid Proteins of HIV-1/HIV-2
Miyazaki, Yasuyuki Tokyo Metropolitan Institute of Medical Science KAKEN Search Researchers
Miyake, Ariko Yamaguchi University KAKEN Search Researchers
Doi, Naoya Tokushima University Tokushima University Educator and Researcher Directory
Koma, Takaaki Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Uchiyama, Tsuneo Tokushima University KAKEN Search Researchers
Adachi, Akio Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Timely disassembly of viral core composed of self-assembled capsid (CA) in infected host cells is crucial for retroviral replication. Extensive in vitro studies to date on the self-assembly/disassembly mechanism of human immunodeficiency virus type 1 (HIV-1) CA have revealed its core structure and amino acid residues essential for CA–CA intermolecular interaction. However, little is known about in vitro properties of HIV-2 CA. In this study, we comparatively analyzed the polymerization properties of bacterially expressed HIV-1 and HIV-2 CA proteins. Interestingly, a much higher concentration of NaCl was required for HIV-2 CA to self-assemble than that for HIV-1 CA, but once the polymerization started, the reaction proceeded more rapidly than that observed for HIV-1 CA. Analysis of a chimeric protein revealed that N-terminal domain (NTD) is responsible for this unique property of HIV-2 CA. To further study the molecular basis for different in vitro properties of HIV-1 and HIV-2 CA proteins, we determined thermal stabilities of HIV-1 and HIV-2 CA NTD proteins at several NaCl concentrations by fluorescent-based thermal shift assays. Experimental data obtained showed that HIV-2 CA NTD was structurally more stable than HIV-1 CA NTD. Taken together, our results imply that distinct in vitro polymerization abilities of the two CA proteins are related to their structural instability/stability, which is one of the decisive factors for viral replication potential. In addition, our assay system described here may be potentially useful for searching for anti-CA antivirals against HIV-1 and HIV-2.
Frontiers in Microbiology
Frontiers Media S.A.
著者英表記誤記あり (誤)Noya Doi→(正)Naoya Doi
Copyright © 2017 Miyazaki, Miyake, Doi, Koma, Uchiyama, Adachi and Nomaguchi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). (https://creativecommons.org/licenses/by/4.0/) The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
|DOI (Published Version)|
|URL ( Publisher's Version )|
fmicb_8_1082.pdf 2.57 MB