ID 113646
Author
Hase, Eiji Tokushima University
Mizuno, Takahiko Tokushima University
Miyamoto, Shuji Tokushima University
Ichikawa, Ryuji Tokushima University
Hsieh, Yi-Da Tokushima University
Shibuya, Kyuki Tokushima University
Nakajima, Yoshiaki The University of Electro-Communications
Asahara, Akifumi The University of Electro-Communications
Minoshima, Kaoru The University of Electro-Communications
Content Type
Journal Article
Description
Confocal laser microscopy (CLM) is a powerful tool in life science research and industrial inspection because it offers two-dimensional optical sectioning or three-dimensional imaging capability with micrometer depth selectivity. Furthermore, scan-less imaging modality enables rapid image acquisition and high robustness against surrounding external disturbances in CLM. However, the objects to be measured must be reflective, absorptive, scattering, or fluorescent because the image contrast is given by the optical intensity. If a new image contrast can be provided by the optical phase, scan-less CLMcan be further applied for transparent non-fluorescent objects or reflective objects with nanometer unevenness by providing information on refractive index, optical thickness, or geometrical shape. Here, we report scan-less confocal dual-comb microscopy offering a phase image in addition to an amplitude image with depth selectivity by using an optical frequency comb as an optical carrier of amplitude and phase with discrete ultra-multichannels. Our technique encodes confocal amplitude and phase images of a sample onto a series of discrete modes in the optical frequency comb with well-defined amplitude and phase to establish a one-to-one correspondence between image pixels and comb modes. The technique then decodes these images from comb modes with amplitude and phase. We demonstrate confocal phase imaging with milliradian phase resolution under micrometer depth selectivity on the millisecond timescale. As a proof of concept, we demonstrate the quantitative phase imaging of standing culture fixed cells and the surface topography of nanometer-scale step structures. Our technique for confocal phase imaging will find applications in three-dimensional visualization of stacked living cells in culture and nanometer surface topography of semiconductor objects.
Journal Title
Optica
ISSN
23342536
Publisher
OSA publishing
Volume
5
Issue
5
Start Page
634
End Page
643
Published Date
2018-05-16
Rights
This is an open access article distributed under a Creative Commons license(https://creativecommons.org/licenses/by/4.0/).
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Science and Technology