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ID 116135
Author
Kinoshita, Chinatsu Tokushima University
Kawase, Tomoki Tokushima University
Sato, Mikio Tokushima University
Sakuraba, Haruhiko Kagawa University
Keywords
pyridoxal 5ʹ-phosphate
amino acid racemase
substrate specificity
Thermococcus litoralis
Content Type
Journal Article
Description
The amino acid sequence of the OCC_10945 gene product from the hyperthermophilic archaeon Thermococcus litoralis DSM5473, originally annotated as γ-aminobutyrate aminotransferase, is highly similar to that of the uncharacterized pyridoxal 5ʹ-phosphate (PLP)-dependent amino acid racemase from Pyrococcus horikoshii. The OCC_10945 enzyme was successfully overexpressed in Escherichia coli by co-expression with a chaperone protein. The purified enzyme demonstrated PLP-dependent amino acid racemase activity primarily toward Met and Leu. Although PLP contributed to enzyme stability, it only loosely bound to this enzyme. Enzyme activity was strongly inhibited by several metal ions, including Co2+ and Zn2+, and non-substrate amino acids such as L-Arg and L-Lys. These results suggest that the underlying PLP-binding and substrate recognition mechanisms in this enzyme are significantly different from those of the other archaeal and bacterial amino acid racemases. This is the first description of a novel PLP-dependent amino acid racemase with moderate substrate specificity in hyperthermophilic archaea.
Journal Title
Bioscience, Biotechnology, and Biochemistry
ISSN
09168451
13476947
NCID
AA10824164
Publisher
Japan Society for Bioscience, Biotechnology, and Agrochemistry|Oxford University Press
Volume
85
Issue
7
Start Page
1650
End Page
1657
Published Date
2021-05-04
Rights
This is a pre-copyedited, author-produced version of an article accepted for publication in Bioscience, Biotechnology, and Biochemistry following peer review. The version of record Ryushi Kawakami, Chinatsu Kinoshita, Tomoki Kawase, Mikio Sato, Junji Hayashi, Haruhiko Sakuraba, Toshihisa Ohshima, Characterization of a novel moderate-substrate specificity amino acid racemase from the hyperthermophilic archaeon Thermococcus litoralis, Bioscience, Biotechnology, and Biochemistry, Volume 85, Issue 7, July 2021, Pages 1650–1657 is available online at: https://doi.org/10.1093/bbb/zbab078.
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language
eng
TextVersion
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departments
Bioscience and Bioindustry