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ID 113338
Title Alternative
レスベラトロールは子宮肉腫細胞において、wntシグナルを抑制することによりアポトーシスを誘導し、細胞増殖を抑制する
Author
Mineda, Ayuka Tokushima University
Takiguchi, Eri Tokushima University
Keywords
resveratrol
uterine sarcoma
Wnt signaling pathway
β-catenin
glycogen synthase kinase-3β
Content Type
Thesis or Dissertation
Description
Resveratrol, a natural product and peroxisome proliferator-activated receptor (PPAR) agonist, has been reported to exert anti-cancer effects in several tumor models. A previous study by our group reported that prostaglandin J2, a PPARγ ligand, inhibited cell proliferation in a uterine sarcoma cell line. The aim of the present study was to investigate the role of the Wnt signaling pathway in resveratrol-induced apoptosis and inhibition of cell proliferation in the MES-SA human uterine sarcoma cell line. A WST-1 assay demonstrated that resveratrol inhibited cell proliferation in the MES-SA cell line, and flow cytometry revealed that the number of apoptotic cells increased in a resveratrol dose-dependent manner. The mechanisms underlying these effects of resveratrol were speculated to involve the expression of β-catenin and its target gene, c-myc, which were examined using western blot analysis. The results revealed a dose-dependent downregulation of this β-catenin and c-myc. This effect was blunted by a pharmacological inhibitor of glycogen synthase kinase 3β. Therefore, it is likely that resveratrol inhibited the cell proliferation and increased the number of apoptotic cells, at least partially, via the Wnt signaling pathway. The present results suggest that resveratrol is a potential candidate for the treatment of uterine sarcoma.
Journal Title
Experimental and Therapeutic Medicine
ISSN
17920981
17921015
NCID
AA12610820
Publisher
Spandidos Publications
Volume
17
Issue
3
Start Page
2242
End Page
2246
Published Date
2019-01-28
Remark
内容要旨・審査要旨・論文本文の公開
本論文は, 著者AYUKA MINEDAの学位論文として提出され, 学位審査・授与の対象となっている。
Rights
© Mineda et al. This is an open access article distributed under the terms of Creative Commons Attribution License.( https://creativecommons.org/licenses/by-nc-nd/4.0/ )
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3254号
Diploma Number
甲医第1403号
Granted Date
2019-03-22
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
University Hospital
Medical Sciences