ID 110791
Author
Otsuka, Toshihiro Department of Digestive and Pediatric Surgery, Institute of Health Biosciences ,The University of Tokushima Graduate School
Izumi, Keisuke Department of Molecular and Environmental Pathology, Institute of Health Biosciences ,The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Tokunaga, Itsuo Department of Legal Medicine, Institute of Health Biosciences ,The University of Tokushima Graduate School KAKEN Search Researchers
Gotohda, Takako Department of Legal Medicine, Institute of Health Biosciences ,The University of Tokushima Graduate School
Ipposhi, Kaneshige Department of Clinical Pharmacology, Institute of Health Biosciences ,The University of Tokushima Graduate School
Takiguchi, Yoshiharu Department of Clinical Pharmacology, Institute of Health Biosciences ,The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kaneda, Shinya Department of Molecular and Environmental Pathology, Institute of Health Biosciences ,The University of Tokushima Graduate School
Satake, Nobuo Department of Molecular and Environmental Pathology, Institute of Health Biosciences ,The University of Tokushima Graduate School
Ohnishi, Takamasa Department of Molecular and Environmental Pathology, Institute of Health Biosciences ,The University of Tokushima Graduate School
Tashiro, Seiki Department of Digestive and Pediatric Surgery, Institute of Health Biosciences ,The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory
Shimada, Mitsuo Department of Digestive and Pediatric Surgery, Institute of Health Biosciences ,The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
LEC rat
8-OHdG
glutathione peroxidase
D-galactosamine hydrochloride
Wilson’s disease
Content Type
Journal Article
Description
Repeated injections of D-galactosamine hydrochloride (GalN) increase the survival rate of Long-Evans Cinnamon (LEC) rats, an animal model of Wilson’s disease. The aim of the present study was to investigate the mechanism of GalN for prevention of spontaneous lethal hepatic injury in LEC rats. MaleLEC rats were given a single subcutaneous injection of 300mg/kg of GalN or vehicle (0.9%NaCl) at 14weeks, and killed at 28 weeks of age. Next, 6-week-old male LEC rats were given weekly subcutaneous injections of 300 mg/kg of GalN or vehicle for 3 or 12 weeks, and their hepatic 8-hydroxydeoxy-2’-guanosine (8-OHdG), glutathione peroxidase (GPX), and catalase activities were measured. None of GalN-treated rats died of hepatic injury (0/12), whereas the mortality rate of control rats given 0.9% NaCl was 17% (2/12). GalN administration for 12 weeks decreased the hepatic 8-OHdG, and GalN administration for either 3 or 12weeks increased the glutathione peroxidase activity. GalN administration increased the serum level of alanine aminotransferase, and accelerated megalocytic degeneration of the hepatocytes. GalN treatment is effective in preventing lethal hepatitis in LEC rats and decrease of oxidative DNA damage by GalN plays an important role in increase of the survival rate.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
53
Issue
1-2
Start Page
81
End Page
86
Sort Key
81
Published Date
2006-02
EDB ID
FullText File
language
eng
TextVersion
Publisher
departments
Pharmaceutical Sciences
Medical Sciences