number of access : ?
number of downloads : ?
ID 114637
Title Alternative
ペルオキシソーム増殖因子活性化受容体アルファノックアウトマウスの絶食時における睡眠変化
Author
Kondo, Yoshitsugu Tokushima University|Tokushima Bunri University
Tanioka, Daisuke Tokushima University
Uguisu, Haruo Tokushima Bunri University
Keywords
PPARα
Sleep
Food deprivation
Ketone bodies
Content Type
Thesis or Dissertation
Description
Peroxisome proliferator-activated receptor alpha (PPARα) is a transcription factor that belongs to the nuclear receptor family and plays an important role in regulating gene expression associated with lipid metabolism. PPARα promotes hepatic fatty acid oxidation and ketogenesis in response to fasting. Because energy metabolism is known to affect sleep regulation, manipulations that change PPARα are likely to affect sleep and other physiological phenotypes. In this study, we examined the role of PPARα in sleep/wake regulation using PPARα knockout (KO) mice. Sleep, body temperature (BT), locomotor activity, arterial pressure (AP) and heart rate (HR) were recorded in KO mice and wild-type (WT) controls under ad libitum-fed conditions and 24-hour food deprivation (FD). KO and WT mice were identical in basal sleep amount, BT, mean AP and HR, although KO mice showed enhanced sleepiness (enhanced EEG slow-wave activity). In response to FD, KO mice showed a large drop in wakefulness and locomotor activity at the end of the dark phase, whereas WT mice did not. Similarly, AP and HR, which were suppressed by FD, decreased more in KO than in WT mice. Compared to WT mice, KO mice showed a reduced concentration of plasma ketone bodies and decreased mRNA expression of the ketogenic enzyme gene Hmgcs2 in the liver and brain under FD conditions. These results suggest that PPARα and/or lipid metabolism is involved in the maintenance of wakefulness and locomotor activity during fasting in mice.
Journal Title
Physiology & Behavior
ISSN
00319384
NCID
AA11537135
AA00352932
Publisher
Elsevier
Volume
214
Start Page
112760
Published Date
2019-11-27
Remark
内容要旨・審査要旨・論文本文の公開
本論文は, 著者Yoshitsugu Kondoの学位論文として提出され, 学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3365号
Diploma Number
甲医第1448号
Granted Date
2020-03-18
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
Medical Sciences