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ID 110108
Title Alternative
Campylobacter jejuni感染はT-84細胞におけるCFTR活性化によるCl⁻分泌亢進を抑制する
Author
Negoro, Sachie The University of Tokushima
Hatayama, Syo The University of Tokushima
Sato, Yuri The University of Tokushima
Matsumoto, Mari The University of Tokushima
Iba, Hitomi The University of Tokushima
Nishikawa, Yoshikazu Osaka City University
Yamasaki, Shinji Osaka Prefecture University
Keywords
Campylobacter jejuni
CFTR
diarrhea
short-circuit current
efflux assay
Content Type
Thesis or Dissertation
Description
Campylobacter jejuni causes foodborne disease associated with abdominal pain, gastroenteritis, and diarrhea. These symptoms are induced by bacterial adherence and invasion of host epithelial cells. C. jejuni infection can occur with a low infective dose, suggesting that C. jejuni may have evolved strategies to cope with the bacterial clearance system in the gastrointestinal tract. The mucosa layer is the first line of defense against bacteria. Mucus conditions are maintained by water and anion (especially Cl-) movement. Cystic fibrosis transmembrane conductance regulator (CFTR) is the main Cl- channel transporting Cl- to the lumen. Mutations in CFTR result in dehydrated secreted mucus and bacterial accumulation in the lungs, and recent studies suggest that closely related pathogenic bacteria also may survive in the intestine. However, the relationship between C. jejuni infection and CFTR has been little studied.
Here, we used an 125I- efflux assay and measurement of short-circuit current to measure Cl- secretion in C. jejuni-infected T-84 human intestinal epithelial cells. The basic state of Cl- secretion was unchanged by C. jejuni infection, but CFTR activator was observed to induce Cl- secretion suppressed in C. jejuniinfected T-84 cells. The suppression of activated Cl- secretion was bacterial dose-dependent and duration-dependent. A similar result was observed during infection with other C. jejuni strains. The mechanism of suppression may occur by affecting water movement or mucus condition in the intestinal tract. A failure of mucus barrier function may promote bacterial adhesion or invasion of host intestinal epithelial cells, thereby causing bacterial preservation in the host intestinal tract.
Journal Title
Journal of Infection and Chemotherapy
ISSN
1341321X
NCID
AA11057978
AA11627318
Publisher
Japanese Society of Chemotherapy and The Japanese Association for Infection Disease|Elsevier
Volume
20
Issue
11
Start Page
682
End Page
688
Published Date
2014-08-06
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨:LID201704261024.pdf
論文本文:LID201704261025.pdf
本論文は, 著者Sachie Negoroの学位論文として提出され, 学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3089号
Diploma Number
甲栄第241号
Granted Date
2017-03-23
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University
departments
Medical Sciences
Bioscience and Bioindustry