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ID 110124
Title Alternative
食餌性リンは大腸炎モデル動物の病態を悪化させる
Author
Sugihara, Kohei Tokushima University
Nakao, Mari Tokushima University
Abuduli, Maerjianghan Tokushima University
Imi, Yukiko Tokushima University
Oda, Naoko Tokushima University
Keywords
Dietary Pi
DSS-induced colitis
proinflammatory cytokine
NF-κB
ROS
Content Type
Thesis or Dissertation
Description
The recent widespread consumption of Western diets and food additives worldwide is associated with excessive inorganic phosphate intake. However, researchers have known little about the impact of dietary phosphate intake on the development of inflammatory bowel disease to date. In this study, we investigated the effects of dietary phosphate on intestinal inflammation in experimental colitis. Sprague-Dawley rats were fed different phosphate diets (0.5%, 1.0% and 1.5% phosphate) with or without dextran sulfate sodium. For in vitro study, the effects of phosphate on proinflammatory cytokine induction and reactive oxygen species production in RAW264.7 macrophage were examined. Dietary phosphate exacerbated intestinal inflammation in experimental colitis in a dose-dependent manner, as assessed by the clinical disease activity score, colon length, and histology. Furthermore, the high phosphate diet increased myeloperoxidase activity and proinflammatory cytokine mRNA expression through the activation of nuclear factor κB in the inflamed colon. In addition, high phosphate loading in RAW264.7 cells directly enhanced reactive oxygen species production and proinflammatory cytokine gene expression. Our results demonstrated that the high phosphate diet exacerbated intestinal inflammation in experimental colitis. These findings have important therapeutic implications for inflammatory bowel disease patients.
Journal Title
Journal of Clinical Biochemistry and Nutrition
ISSN
18805086
09120009
NCID
AA10710201
AA12091064
Publisher
SOCIETY FOR FREE RADICAL RESEARCH JAPAN
Volume
61
Issue
2
Start Page
91
End Page
99
Published Date
2017-09-01
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨:LID201705191027.pdf
論文本文:k3010_fulltext.pdf
著者の申請により要約(2017-05-19公開)に替えて論文全文を公開(2018-02-15)
本論文は, 著者Kohei Sugiharaの学位論文として提出され, 学位審査・授与の対象となっている。
Rights
© 2017 Society for Free Radical Research Japan
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3090号
Diploma Number
甲栄第242号
Granted Date
2017-03-23
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University
departments
Medical Sciences